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Waternut corm strong seedling culture medium and waternut corm seedling field transplanting method

A culture medium and technology of water chestnut seedlings, applied in the direction of culture medium, planting substrate, horticultural methods, etc., can solve the problems of low survival rate of tissue culture seedlings in field transplanting, restrictions on large-scale production of water chestnut tissue culture seedlings, and high production costs. Achieve the effect of being conducive to root growth, easy to apply on a large scale, and easy to operate

Active Publication Date: 2020-02-18
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the process of rapid propagation of water chestnuts, there are problems such as low multiplication coefficient and slow proliferation speed, severe browning of materials after cutting, low survival rate of tissue culture seedlings transplanted in field, and high production costs, which restrict the scale of water chestnut tissue culture seedlings. chemical production

Method used

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  • Waternut corm strong seedling culture medium and waternut corm seedling field transplanting method
  • Waternut corm strong seedling culture medium and waternut corm seedling field transplanting method

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The preparation method of water chestnut strong seedling medium: accurately weigh 4.74g of MS medium dry powder, 30g of sucrose, first heat the accurately measured 1000ml deionized water to boiling, then accurately weigh 4.74g of MS medium dry powder, sucrose 30g was added sequentially, and after the two were completely dissolved, KNO was added to it respectively 3 and (NH 4 ) 2 SO 4 , keeping the ratio of the amount and concentration of its substances at 1:4, adjusting the pH to 5.8, KNO 3 and (NH 4 ) 2 SO 4 The concentration treatment is 1000ml, repeats 4 times. 116°C high pressure steam sterilization for 30min.

[0021] Using water chestnut tissue culture seedlings as material, cut the proliferated cluster buds to grow 2.5-3.0cm, take 3-4 leaf-like stems as a cluster, inoculate in the water chestnut strong seedling medium for strong seedling cultivation, and set the culture temperature at 23~27℃, the light intensity is 30μmol·m -2 ·s -1 , the light time is ...

Embodiment 2-3

[0023] The difference between embodiment 2-3 and embodiment 1 is: KNO 3 and (NH 4 ) 2 SO 4 The ratios of the amount and concentration of the substances are 1:2 and 1:3, respectively.

Embodiment 4

[0034] A method for field transplanting of water chestnut seedlings, comprising the following steps:

[0035] (1) Artificial planting of water chestnut seedlings: unscrew the tissue culture bottle caps of the strong water chestnut seedlings cultivated by strong seedlings, remove the bottle caps after performing environmental adaptability exercise in the culture room for 7 days, move the tissue culture seedlings out of the room after 30 days Take it out from the culture bottle, change the water once every 5d in 30d, rinse the culture medium with clear water, transplant it in the cultivation medium, and the cultivation medium is garden soil and commercial substrate (the commercial product adopted in this embodiment) with a volume ratio of 1:1. The substrate is composed of Xingxing Xiangnong brand seedling special substrate produced by Jiangsu Xingnong Substrate Technology Co., Ltd.;

[0036] (2) Transplant the surviving water chestnut tissue culture seedlings in the step (1) to ...

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Abstract

The invention discloses a waternut corm strong seedling culture medium and a waternut corm seedling field transplanting method. The waternut corm strong seedling culture medium comprises, by weight, 4-5 parts of MS culture medium dry powder, 30-40 parts of sucrose, 0.4-0.6 part of KNO3, 2-4 parts of (NH4)2SO4 and 800-1000 parts of deionized water. The waternut corm seedling field transplanting method includes the steps: unscrewing a tissue culture bottle cap for healthy and strong waternut corm seedlings cultured by the waternut corm strong seedling culture medium, performing environmental adaptation training in a culture room for 7 days and then removing the bottle cap, transferring the seedlings into an indoor environment, placing the seedlings in the indoor environment for 30 days, taking out the tissue culture seedlings from a culture bottle, changing water once every 5 days within 30 days, washing out the culture medium with clean water and transplanting the seedlings into a culture substrate; transplanting the waternut corm tissue culture seedlings surviving in provisional planting in the step (1) into a field. By the aid of the culture medium, the waternut corm tissue culture seedlings can generate root systems in the seedling strengthening process, independent rooting culture steps are omitted, and field transplanting survival rate can be increased by the field transplanting method.

Description

technical field [0001] The invention relates to a culture medium for strengthening seedlings of water chestnuts, in particular to a culture medium for strengthening seedlings of water chestnuts and a method for field transplanting of water chestnut seedlings. Background technique [0002] Water chestnut [Eelocharis tuberosa (Roxb.) Roem.et Schult.] is a perennial shallow-water plant of Cyperaceae (Cyperaceae) water chestnut (Eleocharis R Br.), also known as horseshoe, ground chestnut, scorpion, black taro, etc., native to In southern my country and India, there have been more than 2,000 years of cultivation history in our country. Water chestnuts are sweet and juicy, crispy and refreshing, and are one of the important aquatic vegetables with both medicinal and edible fruits and vegetables. They have rich nutritional and health value It also has the effects of clearing away heat, reducing phlegm and eliminating accumulation. At the same time, water chestnuts can be processed in...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G22/25A01G24/10
CPCA01H4/001A01H4/008A01G22/25A01G24/10
Inventor 马绍鋆俞飞飞严从生董言香王明霞王艳贾利江海坤
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI