Knoxia breeding method

A technology of red bud spurge and light intensity, applied in the field of plant reproduction, can solve the problems of less than 1% seed germination rate, low seed viability and germination rate, waste of medicinal materials, etc., achieves a stable and high-efficiency tissue culture system, reduces breeding cost, the effect of increasing the reproductive rate

Inactive Publication Date: 2020-02-21
赵健华 +2
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  • Description
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Problems solved by technology

[0003] Euphorbia is mainly propagated by seeds. Under natural conditions, the viability and germination rate of its seeds are low. Because the seeds are not fully mature or the embryos are not fully developed, the germination rate of the seeds scattered on the ground is less than 1%, and The germination rate of seeds decreases after 1 year of storage time, and the old seeds stored for more than 1 year by conventional methods basically lose their vitality and hardly emerge.
[0004] At present, the efficiency of Euphorbia euphorbius using natural zongzi to reproduce is low, and most of the vegetative propagation is carried out with medicinal parts-roots, which will reduce its medicinal raw materials to a certain extent, resulting in waste of medicinal materials. If it is less than required, it will accelerate the extinction of wild resources

Method used

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  • Knoxia breeding method
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Embodiment 1

[0025] A method for breeding Euphorbia euphorbia, comprising the following steps:

[0026] 1) Sterilization of explants: Take the vigorously growing Euphorbia euphorbia plant, take the stalk, first sterilize it with 70% alcohol for 1 min, wash it 3 times with sterile water, then sterilize it with 10% sodium hypochlorite solution for 15 min, and use sterile Rinse with bacterial water 5 times.

[0027] 2) Callus induction culture: Cut the stalks treated in step 1) into 1.5±0.2cm long stem segments, and then square them in the induction medium for 10 days. The induction medium is: MS+1.5mg / L KT+0.5mg / L 6-BA+20g / L sucrose+7.5g / L agar;

[0028] The culture conditions are as follows: the culture temperature is 26°C, the light time is 13h / d, and the light intensity is 1200Lx.

[0029] 3) Callus differentiation culture: Inoculate callus with good growth state into differentiation medium and continue to cultivate for 28 days. The differentiation medium is: MS+0.1mg / L KT+0.5mg / L 6-BA+...

Embodiment 2

[0037] A method for breeding Euphorbia euphorbia, comprising the following steps:

[0038] 1) Sterilization of explants: Take the vigorously growing Euphorbia euphorbia plant, take the stalk, first sterilize it with 70% alcohol for 1 min, wash it 4 times with sterile water, then sterilize it with 10% sodium hypochlorite solution for 15 min, and use sterile Rinse with bacterial water 6 times.

[0039] 2) Callus induction culture: Cut the stalks treated in step 1) into 1.5±0.2cm long stem segments, and then square them in the induction medium for 12 days. The induction medium is: MS+1g / L KT +0.3mg / L 6-BA+20g / L sucrose+7.5g / L agar;

[0040] The culture conditions are as follows: the culture temperature is 24° C., the light time is 15 h / d, and the light intensity is 800 Lx.

[0041] 3) Callus differentiation culture: Inoculate the callus with good growth state into the differentiation medium and continue to cultivate for 28 days. The differentiation medium is: MS+0.5mg / L KT+0.5m...

Embodiment 3

[0049] A method for breeding Euphorbia euphorbia, comprising the following steps:

[0050] 1) Sterilization of explants: Take the vigorously growing Euphorbia euphorbia plant, take the stalk, first sterilize it with 70% alcohol for 1 min, wash it 3 times with sterile water, then sterilize it with 10% sodium hypochlorite solution for 15 min, and use sterile Rinse with bacterial water 6 times.

[0051] 2) Callus induction culture: cut the stalks treated in step 1) into 1.5±0.2cm long stem segments, and then square them in the induction medium for 12 days. The induction medium is: MS+0.5mg / L KT+0.6mg / L 6-BA+20g / L sucrose+7.5g / L agar;

[0052] The culture conditions are as follows: the culture temperature is 25° C., the light time is 15 h / d, and the light intensity is 1000 Lx.

[0053] 3) Callus differentiation culture: Inoculate the callus with good growth state into the differentiation medium and continue to cultivate for 30 days. The differentiation medium is: MS+0.3mg / L KT+1...

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Abstract

The invention provides a knoxia breeding method, which includes the following steps: (1) sterilizing an explant: taking a stem of a knoxia plant, disinfecting with alcohol, washing with sterile water,disinfecting with a sodium hypochlorite solution, and washing with sterile water; (2) callus induction culture: cutting the stem into stem segments, wherein the induction medium is MS + 0.5-1.5mg/L of KT + 0.3-0.6mg/L of of 6-BA + 20g/L of sucrose + 7.5g/L of agar; (3) callus differentiation culture: wherein the differentiation medium is MS + 0.1-0.5mg/L of KT + 0.5-1mg/L of 6-BA + 20g/L of sucrose + 7.5g/L of agar; (4) subculture: wherein the cultrue medium is MS + 0.5-1mg/L of NAA + 1-2mg/L of 6-BA + 0.5-1mg/L of 2,4-D + 25g/L of sucrose + 7.5g/L of agar; (5) rooting culture: wherein the culture medium for rooting culture is 1/2 MS + 0.3-0.6mg/L of IBA + 20g/L of sucrose + 5g/L of agar; and (6) transplanting. The knoxia breeding method can quickly breed knoxia seedlings with excellent quality and can reduce the breeding cost of the knoxia.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a breeding method of Euphorbia euphorbia. Background technique [0002] Red Euphorbia is a Rubiaceae plant, which belongs to rare Chinese medicinal materials, also known as Red Euphorbia. The medicinal root has the effects of draining water and drinking, reducing swelling and dissipating stagnation. It is a small variety of Chinese medicinal materials, with an annual demand of about 150 tons. Wild resources are distributed in Guangdong, Guangxi, Hainan, Fujian, and Yunnan. It is also distributed in Indochina Peninsula and India. [0003] Euphorbia is mainly propagated by seeds. Under natural conditions, the viability and germination rate of its seeds are low. Because the seeds are not fully mature or the embryos are not fully developed, the germination rate of the seeds scattered on the ground is less than 1%, and The germination rate of the seeds decreased after 1 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008A01H4/005
Inventor 赵健华杨楠杨发建
Owner 赵健华
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