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Cell transport protection solution, preparation method and application

A technology for protecting liquid and cells, applied in the biological field, can solve the problems of insecure research experiments, inability to directly observe the state, affecting the survival rate of cells, etc., to maintain a sterile growth environment, suitable for large-scale production applications, and good bacteriostasis and sterilization. effect of action

Active Publication Date: 2021-07-09
KYBIOSTEM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, in order to ensure the activity of mesenchymal stem cells during the transportation process, the cells are usually frozen and then transported with liquid nitrogen or dry ice. The transportation conditions are very harsh. It is necessary to ensure that the whole transportation is in a low temperature state, and the cost is high.
And if the cells are repeatedly frozen and thawed during transportation, the cell viability will be affected
In addition, the state of the received cells cannot be directly observed, and there is no guarantee for subsequent research experiments

Method used

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  • Cell transport protection solution, preparation method and application
  • Cell transport protection solution, preparation method and application
  • Cell transport protection solution, preparation method and application

Examples

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preparation example Construction

[0066] The preparation method of the cell transport protection solution provided by the invention has the advantages of simple process and convenient operation. The cell transport protection solution can be obtained as long as the protection agent, basal medium, albumin, antibiotics and water are dissolved and mixed uniformly. The preparation method does not require expensive instruments, can effectively save costs, and is suitable for large-scale production applications.

[0067] In some specific embodiments, taking 1L of cell transport protection solution as an example, under sterile conditions, take 0.1-0.3L of basal medium and add 0.01-0.03L of albumin, and then add 2-15g of protective agent and 0.2g of antibiotics -1.5g, after stirring and dissolving, add sterile deionized water to a constant volume of 1L, and stir evenly to obtain a cell transport protection solution.

[0068] In addition, the present invention also provides the application of the above-mentioned cell tr...

Embodiment 1

[0071] This example provides a cell transport protection solution. Each 1L of the cell transport protection solution includes: 5g of hydroxypropyl cellulose, 0.2L of MEM, 0.015L of fetal bovine serum, 0.2g of penicillin, 0.2g of streptomycin and more amount of water.

Embodiment 2

[0073] This example provides a cell transport protection solution. Each 1L of the cell transport protection solution includes: 10 g of low-viscosity carboxymethyl cellulose sodium salt, 0.15 L of MEM, 0.025 L of fetal bovine serum, 0.5 g of penicillin, and 0.5 g of streptomycin 0.5g and the balance of water.

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PUM

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Abstract

The invention provides a cell transport protection solution and preparation method and application, which involves the field of biotechnology. The cell transport protective liquid provided by the present invention includes protective agents, basic medium, white protein, antibiotics and water.Among them, the protective agent includes cellulose, cyclum, polymer, or Polosham 188. The above substances are used as protective agents. The cost is low, and it can slow down the growth rate of cells, prevent cell growth too dense, and affect cell activity.In addition, the above protective agent can also increase the viscosity of cell transportation protective liquids, effectively avoiding cell physical damage caused by vibration and shaking during transportation.At the same time, the basic medium is rich in essential components of cell metabolism, and albumin can also provide nutrition for cells, thereby effectively maintaining cell activity.In addition, antibiotics can play a good role in bacteriostatic bactericidal and maintain a sterile growth environment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cell transport protection solution and a preparation method and application. Background technique [0002] With the development of medicine, in the process of clinical application and scientific research practice, people's demand for the preservation of isolated organs, tissues and cells is constantly increasing, and the standards are constantly improving. In the process of preservation and transportation of some organs or tissues to be transplanted , and also put forward higher requirements on the medium for cell survival. It is not only necessary to ensure the viability of organs, tissues or cells, but also to retain their original biological activities to the greatest extent, and at the same time to prevent them from being polluted by bacteria and to prevent their death. The properties of various organs, tissues or cells are different, and their storage media also have differen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0226
Inventor 焦晓敏杜宏武
Owner KYBIOSTEM CO LTD
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