Azole eleostearate derivative with anticancer activity
A technology of cartilic acid azole and anticancer activity, which is applied in the field of compounds, can solve the problems of destroying cell growth, application limitations, toxic and side effects, etc., and achieve the effect of simple operation of the method, broadening the scope, and increasing anticancer activity
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Embodiment 1
[0022] The following examples are some examples of the present invention, and should not be regarded as limiting the present invention.
[0023] Preparation of citric acid chloride: Weigh citric acid and thionyl chloride at a molar ratio of 1:3, dissolve civic acid in 60m methylene chloride, slowly add thionyl chloride dropwise, and react at 20°C for 2 hours. After the reaction is over, the steric acid chloride is obtained by distillation under reduced pressure. Its structure is as follows:
[0024]
[0025] Preparation of citric acid azole derivatives: Weigh the raw material 3-amino-5-mercapto-1,2,4-triazole and dissolve it in 50 mL of dichloromethane solution. After slowly adding citric acid chloride at a molar ratio of 1:1, the reaction was started under reflux at -10°C for 2 hours. Thin-layer chromatography tracking detection, after the reaction is complete, add an appropriate amount of water to wash 3 times. Add dichloromethane, shake well and extract, remove the lo...
Embodiment 1
[0027] Cartilic acid nitrogen azole derivative 1H NMR (MHz, CDCl in embodiment 1 3 ,δppm): δ0.96~2.23(-CH 3 ,-CH 2 -); δ5.65~6.51(-CH=CH-); δ7.01~7.65(-CH-); δ3.0(-SH); δ4.0(-NH 2 ). IR:2915,2849cm -1 (-CH 2 ,-CH 3 );1727cm -1 (C=O); 1553cm -1 (N–H); 1169cm -1 、1140cm -1 (N–C).
Embodiment 2
[0029] Preparation of citric acid chloride: weigh citric acid and oxalyl chloride at a molar ratio of 1:4, dissolve citric acid in 60mM dichloromethane, slowly add thionyl chloride, and react at 70°C for 3 hours. After the reaction is over, the steric acid chloride is obtained by distillation under reduced pressure. Its structure is as follows:
[0030]
[0031] Preparation of citric acid azole derivatives: Weigh the raw material 3,5-dibromo-1,2,4-triazole and dissolve it in 50 mL of dichloromethane solution. After slowly adding citric acid chloride at a molar ratio of 1:2, the reaction was started under reflux at 0°C for 2 hours. Thin-layer chromatography tracking detection, after the reaction is complete, add an appropriate amount of water to wash 3 times. Add dichloromethane, shake well and extract, remove the lower layer of liquid under reduced pressure to remove the solvent, and dry over anhydrous sodium sulfate. Add absolute ethanol to the obtained crude product at...
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