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Irritable bowel syndrome related flora marker and kit thereof

A technology for irritable bowel syndrome and markers, applied in the field of biomarkers, can solve the problems of rapid and accurate diagnosis of IBS that are not used to deterministically distinguish IBS, so as to reduce pain, improve the ease of promotion, and facilitate transportation. Effect

Pending Publication Date: 2020-02-25
康美华大基因技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, because the symptoms of IBS are similar or identical to those of many other bowel conditions, distinguishing the similarity of symptoms between IBS and other diseases or disorders can be very challenging and can take years to make a correct diagnosis
For example, patients with inflammatory bowel disease (IBD) who show mild signs and symptoms such as gas, diarrhea, constipation, and abdominal pain may be difficult to distinguish from those with IBS
Unfortunately, there are currently no rapid and accurate diagnostic methods for definitively differentiating IBS from other bowel diseases or disorders presenting with similar symptoms

Method used

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  • Irritable bowel syndrome related flora marker and kit thereof
  • Irritable bowel syndrome related flora marker and kit thereof
  • Irritable bowel syndrome related flora marker and kit thereof

Examples

Experimental program
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Embodiment 1

[0069] Example 1: Establishment of an evaluation method for irritable bowel syndrome

[0070] 1) Sample collection and DNA extraction

[0071] 330 subjects (recorded as the first group in the full text), including 264 patients with irritable bowel syndrome and 66 healthy control subjects, as shown in Table 1 below, the stool samples were collected from Hong Kong Baptist University, and all were informed agree.

[0072] To qualify for this study, participating individuals were required to meet the following criteria prior to collection of stool samples:

[0073] a) Not taking antibiotics, probiotics, prebiotics or microbial supplements for at least three weeks;

[0074] b) Healthy volunteers have no recent history of treatment, cardiovascular disease, intestinal disease, metabolic imbalance, or recent gastrointestinal surgery.

[0075] The subjects' fresh stool samples were stored on dry ice, and immediately placed in a -80°C refrigerator for later use after delivery to th...

Embodiment 2

[0094] Example 2: Validation of Irritable Bowel Syndrome Assessment Method

[0095] From the 17 flora markers obtained in Example 1, the inventor collected another new research group (hereinafter referred to as the second group) to verify the identification ability of the irritable bowel syndrome classifier. The cohort included 86 IBS patients and 15 healthy controls, also from Hong Kong Baptist University. For each sample, the methods described in Example 1 were used for DNA extraction, DNA library construction, and high-throughput sequencing. The inventor obtained the species abundance profiles of these samples through Metaphlan2 software, and then obtained the relative abundance of each species indicated by TAX ID 1-17. In this evaluation analysis, the probability of suffering from irritable bowel syndrome is then calculated for each sample using a random forest algorithm. Among the 38 samples that were greater than the critical value of 0.8, there were 35 patients with...

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Abstract

The invention discloses an irritable bowel syndrome related flora marker which can be used for developing a disease risk assessment system. According to the invention, an optimal set of 17 irritable bowel syndrome related flora markers is obtained, and a kit constructed based on the optimal set is used for diagnosing irritable bowel syndrome and can achieve higher accuracy.

Description

technical field [0001] The invention relates to the technical field of biomarkers, in particular to markers of irritable bowel syndrome-related flora and a kit thereof. Background technique [0002] Irritable bowel syndrome (Irritable bowel syndrome or IBS) is a common intestinal dysfunction, which affects more than 10% of the world's population. IBS can be divided into diarrhea, constipation, mixed and other types. In addition to phenotypic changes related to defecation, such as abdominal pain, diarrhea, constipation or alternating diarrhea and constipation, abdominal distension, flatulence, and excessive fecal mucus, many patients will also show symptoms of depression, so IBS is often described as a "brain-gut syndrome." disease". Although the etiology of IBS has not been fully identified, a growing body of research suggests that alterations in the gut microbiota are closely associated with it. [0003] In current clinical practice, the diagnosis of irritable bowel synd...

Claims

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Application Information

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IPC IPC(8): G16H50/30G16B30/00C12Q1/689C12Q1/04C12Q1/6869G06N3/00
CPCG16H50/30G16B30/00C12Q1/689C12Q1/6869G06N3/006C12Q2535/122
Inventor 许冬瑾杨超周勇熊腾
Owner 康美华大基因技术有限公司
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