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Method for anti-inflammation of skin and promoting keratinocyte proliferation

A technology of keratinocytes and Wedelia, which is applied in the field of anti-skin inflammation and promotion of keratinocyte hyperplasia, the extract of South American Wedelia, can solve the problems of high price, harmfulness to human health, etc., achieve anti-skin inflammation, improve skin protection, promote Rapid update and proliferative effects

Inactive Publication Date: 2020-03-03
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the known medicines and skin care products are made of chemical ingredients. Long-term use is not only harmful to human health, but also these products are often expensive, which is not affordable for ordinary users

Method used

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  • Method for anti-inflammation of skin and promoting keratinocyte proliferation
  • Method for anti-inflammation of skin and promoting keratinocyte proliferation
  • Method for anti-inflammation of skin and promoting keratinocyte proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1. Preparation of the extract of Wedelia chinensis

[0037] First, the whole plant of Wedelia chinensis (the source is harvested by farmers) is washed for subsequent extraction. Next, take the washed whole Wedelia chinensis and homogenize it, then use water or alcohols as the extraction solvent for the homogenate; The liquid-solid ratio is extracted at 50-100° C. for 0.5-3 hours. Afterwards, cool to room temperature and filter through a 400-mesh filter, and then concentrate the filtered product under reduced pressure at 45-70° C. to obtain an extract of Wedelia chinensis.

Embodiment 2

[0038] Example 2. Efficacy evaluation of the extract of Wedelia chinensis in anti-skin inflammation

[0039] Since it is known that interleukin-8 (IL-8) is a cytokine, it has the function of promoting inflammation. When an inflammation occurs, the inflamed tissue will release specific cytokines to attract specific functional Cells to specific tissues, such as IL-1, IL-6 and IL-8, among which IL-8 plays an important role in the chemotaxis of neutrophils. First, human primary epidermal keratinocytes (human primary epidermal keratinocyte, HPEKp) were used for skin keratinocyte hyperplasia experiments. The human primary skin keratinocytes were purchased from CELLnTEC (Switzerland) No. HPEK-50. The cells were cultured in serum-free keratinocyte medium (keratinocyte-SFM) (Gibco, USA, number #10724-011). Add 500 μL of medium to each well of a 24-well culture plate, so that each well has 5x10 4 cells. After 24 hours of incubation at 37°C, the medium was removed.

[0040] Afterwar...

Embodiment 3

[0044] Example 3. Evaluation of the effectiveness of the extract of Wedelia chinensis in promoting keratinocyte proliferation

[0045] First, primary human skin keratinocytes were cultured in serum-free keratinocyte medium (keratinocyte-SFM) (Gibco, USA, No. #10724-011). A medium was added to each well of a 96-well culture plate so that each well had 3,000 cells, followed by culturing at 37° C. for 2 hours.

[0046]Afterwards, the primary human skin keratinocytes were divided into three groups, including a control group and two experimental groups (ie, experimental group 1 and experimental group 2). Add 10 μL of 100 μM BrdU labeling reagent (labeling reagent) (Roche; 11647229001) to each group, and add 0.03125 mg / mL and 0.0625 mg / mL extracts of Wedelia chinensis according to the above Example 1 to experimental group 1 and experimental group 1 respectively. Cells in Group 2 were then incubated for 24 hours. Afterwards, the supernatant was removed and 200 μL of fix solution (F...

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Abstract

The present invention relates to new application of phytoextraction, especially to a method for anti-inflammation of skin and promoting keratinocyte proliferation by adopting the abstract of wedelia trilobata. The method comprises the steps of: adopting an extraction process to extract a lot of efficient substances (such as phenolic acids and diterpenes) of the wedelia trilobata, wherein the efficient substances have the functions of keratinocyte rapid updating and hyperplasia and anti-inflammation of skin, so that skin is prevent from being dirty by isolated from the outside, and the protection of the skin is improved.

Description

technical field [0001] The present invention relates to a new use of plant extracts, in particular to the use of a South American wedelia (Sphagneticolatrilobata) extract for anti-skin inflammation and promotion of keratinocyte proliferation. Background technique [0002] Skin inflammatory response (inflammatory response) refers to a series of vascular and cellular responses caused by injury or infection. Although moderate skin inflammatory response can protect the skin from foreign substances, excessive inflammation will cause persistent redness, Symptoms such as swelling, heat, pain, etc., such as allergies or redness of the skin. Existing anti-inflammatory drugs such as nonsteroidal anti-inflammatory drugs (nonsteroidal anti-inflammatory drugs, NSAIDs), although can be used to treat or slow down many skin inflammatory reactions, however, these artificial compounds are still accompanied by varying degrees of side effects, resulting in troubles of those. [0003] In addit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P17/00A61P29/00A61K8/9789A61Q19/00A61Q17/00A61Q17/04
CPCA61K36/28A61Q17/00A61Q17/04A61Q19/00A61K8/9789A61P17/00A61P29/00A61K9/0014A61K2300/00
Inventor 林咏翔
Owner TCI CO LTD
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