GLUT12 protein inhibitor and preparation method and application thereof

A protein inhibitor, injection technology, applied in the field of medicine, can solve the problem that the expression of GLUT12 is not very clear

Inactive Publication Date: 2020-03-10
吴昱晔
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the expression of GLUT12 in bladder cancer tumor tissue is not very clear, and there is no GLUT12 inhibitor available

Method used

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  • GLUT12 protein inhibitor and preparation method and application thereof
  • GLUT12 protein inhibitor and preparation method and application thereof
  • GLUT12 protein inhibitor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 The GLUT12 protein inhibitor of the present invention was prepared with the following components: 0.5% cisplatin, 3% curcumin, 3% lecithin for injection, 5% soybean oil for injection, 0.1% sodium sulfite, and 20% glycerin , Tween-809%, water for injection is added to 100% mass ratio;

[0051] (1) Preparation of the oil phase: add lecithin for injection and Tween-80 to the soybean oil for injection respectively, stir to dissolve it, and use it as the oil phase;

[0052] (2) Preparation of colostrum: Add cisplatin, curcumin and sodium sulfite into water, stir to dissolve them, then add the oil phase prepared in (1) into water, and disperse at high speed shear (speed: 15000 / min, dispersion time :30min), form colostrum;

[0053] (3) High-pressure homogenization: adjusting step (2) to obtain pH 7.2 of the colostrum, and high-pressure homogenization to obtain essence milk;

[0054] (4) Sterile filtration: the refined milk is sterilized by filtration through a 0.22...

Embodiment 2

[0055] Example 2 The GLUT12 protein inhibitor of the present invention was prepared with the following components: cisplatin 0.1%, curcumin 1%, lecithin for injection 1%, soybean oil for injection 35%, sodium sulfite 0.2%, glycerol 15% , Tween-805%, water for injection is added to 100% mass ratio;

[0056] (1) Preparation of the oil phase: add lecithin for injection and Tween-80 to the soybean oil for injection respectively, stir to dissolve it, and use it as the oil phase;

[0057] (2) Preparation of colostrum: Add cisplatin, curcumin and sodium sulfite into water, stir to dissolve them, then add the oil phase prepared in (1) into water, and disperse at high speed shear (speed: 15000 / min, dispersion time :30min), form colostrum;

[0058] (3) High-pressure homogenization: adjusting the pH of the colostrum obtained in step (2) to be 7.2, and high-pressure homogenization to obtain essence milk;

[0059] (4) Sterile filtration: the refined milk is sterilized by filtration throu...

Embodiment 3

[0060] Example 3 The GLUT12 protein inhibitor of the present invention was prepared with the following components: cisplatin 0.2%, curcumin 2%, lecithin for injection 2%, soybean oil for injection 25%, sodium sulfite 0.3%, glycerol 18% , Tween-806%, water for injection is added to 100% mass ratio;

[0061] (1) Preparation of the oil phase: add lecithin for injection and Tween-80 to the soybean oil for injection respectively, stir to dissolve it, and use it as the oil phase;

[0062] (2) Preparation of colostrum: Add cisplatin, curcumin and sodium sulfite into water, stir to dissolve them, then add the oil phase prepared in (1) into water, and disperse at high speed shear (speed: 15000 / min, dispersion time :30min), form colostrum;

[0063] (3) High-pressure homogenization: adjusting the pH of the colostrum obtained in step (2) to be 7.0, and high-pressure homogenization to obtain essence milk;

[0064] (4) Sterile filtration: the refined milk is sterilized by filtration through...

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Abstract

The invention discloses a GLUT12 protein inhibitor and a preparation method and application thereof. The GLUT12 protein inhibitor comprises 0.1%-1.0% of cis-platinum, 0.8%-8% of curcumin, 1%-3% of lecithin for injection, 5%-35% of soybean oil for injection, 0.1%-0.3% of sodium sulfite, 15%-20% of glycerol, 5%-9% of tween-80 and the balance of water for injection. The curcumin component in the GLUT12 protein inhibitor can effectively inhibit the expression of the modified protein, and the curcumin component is combined with cis-platinum in the components, so that the anti-bladder cancer activity of the medicine can be remarkably improved. The GLUT12 protein inhibitor disclosed by the invention has the characteristics of low cost, low toxicity, good stability and the like, and can be used for preparing bladder cancer chemotherapeutic drugs.

Description

technical field [0001] The invention relates to a protein inhibitor, in particular to a GLUT12 protein inhibitor and its preparation method and application, belonging to the technical field of medicine. Background technique [0002] Bladder cancer refers to malignant tumors that occur on the bladder mucosa. It is the most common malignant tumor of the urinary system and one of the top ten common tumors in the whole body, accounting for the first place in the incidence of genitourinary system tumors in my country. [0003] The commonly used chemotherapy regimens for bladder cancer include M-VAP (methotrexate + vinblastine + doxorubicin + cisplatin), GC (gemcitabine + cisplatin) and MVP (methotrexate + vinblastine + cisplatin). ) program, the effective rate of chemotherapy is 40% to 65%. Among the commonly used chemotherapy regimens, cisplatin is the primary chemotherapeutic drug for the treatment of bladder cancer. However, long-term use of this drug can cause drug resistan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/243A61P35/00A61K31/12
CPCA61K31/12A61P35/00A61K33/243A61K2300/00
Inventor 吴昱晔
Owner 吴昱晔
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