Equal-difference dose gradient radiation lung cancer surviving or resisting cell model as well as construction and application

Abstract

The invention discloses a lung cancer resisting cell strain for radiotherapy, and a construction method and an application of the cell strain, and particularly discloses an application of the cell strain in preparation of the equal-difference dose gradient radiation human lung cancer surviving or resisting cell model. The lung cancer cell resisting line for radiotherapy is selected from three radiation resistant cell lines derived from a human lung cancer cell line A549, the strain is named as a human lung cancer cell radioactive ray resistant strain A549-20F, A549-30F and A549-40F respectively, and the preservation numbers of the strains are CGMCC No. 18161, CGMCC No. 18163 and CGMCC No. 18162 respectively. The cell model provided by the invention is closer to a clinical dose segmentationmodel, the radiation effect is more closely observed and researched dynamically according to the radiation dose in clinical practice; a series of continuous specific changes caused by progressive increase of the radiotherapy dosage in a lung cancer patient can be better simulated, key biomarkers for promoting lung cancer radiation survival / resistance can be found, a radiation resistance generation mechanism can be clarified, and the model has important significance for clinically optimizing a radiotherapy scheme and realizing accurate radiotherapy.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to a radiotherapy-resistant lung cancer cell line, as well as a construction method and application of the cell line, in particular to the application of the cell line in the preparation of a constant dose gradient radiation survival or resistance human lung cancer cell model. Background technique [0002] Lung cancer still ranks first in the morbidity and mortality of malignant tumors worldwide. With the recognition of the role of active local therapy in advanced lung cancer in recent years, and the determination of the immune activation effect of radiotherapy in clinical practice, radiotherapy is playing an increasingly important role in the overall management of lung cancer. However, even given radical concurrent radiotherapy and chemotherapy, there is still a 30%-50% local failure rate, and it is reported that 79% of local recurrences are recurrences in the irradiation field. The safe imp...

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Problems solved by technology

[0004] Aiming at the lack of ability to dynamically observe and detect the resistance of lung cancer cells to radiation in the process of increasing the dose of radiotherapy in the prior art, the present invention provides a radiotherapy-resistant lung cancer cell line

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