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Method for recognizing amino acid enantiomers by adopting chiral gold nano-channel

A gold nanochannel and nanochannel technology, which is applied in the direction of material analysis, measurement device, and instrument by electromagnetic means, can solve problems such as toxicity and differences in drug effects, and achieve the effect of efficient identification

Inactive Publication Date: 2020-03-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These chiral amino acids are very similar in structure and chemical and physical properties, but have great differences in toxicity and drug effects

Method used

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  • Method for recognizing amino acid enantiomers by adopting chiral gold nano-channel
  • Method for recognizing amino acid enantiomers by adopting chiral gold nano-channel
  • Method for recognizing amino acid enantiomers by adopting chiral gold nano-channel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Electrochemical testing of chiral nanochannels

[0028] The prepared chiral nanochannel (specifically as figure 1 and figure 2 as shown, figure 1 is a macroscopic photo of the chiral nanomembrane channel, figure 2 is the circular dichroism spectrum of the chiral nanofilm) placed in the middle of two plexiglass test sample cells with a volume of 2mL, and the bottom of the cube used has a hole with a diameter of 1mm for connecting the two electrochemical test sample cells. Place the prepared chiral nanochannel in the middle of the two test sample pools.

[0029] Add the same 2 mL of PBS solution into the two sample pools, the concentration of the PBS solution is 10 mM. Put two silver / silver chloride electrodes into two sample cells respectively, and then use the linear scan mode of the electrochemical workstation to conduct the current-voltage curve test. The applied voltage is -2V~+2V, and the scanning speed is 20 mV / s. Through this method, the I-U curv...

Embodiment 2

[0030] Example 2 Identification of Amino Acid Enantiomers

[0031] In the two sample pools of the electrochemical test, in addition to 2mL PBS solution with a concentration of 10mM, an L- or D-type chiral amino acid molecular aqueous solution was added to the two sample pools at the same time, so that the final concentration of the amino acid in the PBS solution The concentration is 1 mM. After adding the amino acid solution, the whole system was incubated at room temperature for 10 min before electrochemical testing. Taking L-tryptophan as an example, the electrochemical curve was tested before amino acid was added, and the rectification ratio was 1; after adding amino acid and incubated for 10 minutes, the electrochemical curve was tested, and the rectification ratio was 7.48. Electrochemical test results such as image 3 shown.

Embodiment 3

[0032] Example 3 Calculation of Amino Acid Enantiomer Recognition Strength

[0033] The I-U curve is obtained by electrochemical test, and the recognition strength of different amino acid enantiomers is confirmed by the calculation of rectification ratio. The specific calculation method is: the ratio obtained by dividing the corresponding current value under the -2V scanning voltage by the corresponding current value under the +2V scanning voltage.

[0034] The rectification ratio statistics of different amino acid enantiomers in L- and D-phenylalanine-modified chiral gold nanochannels are listed in Figure 4 and Figure 5 . Figure 4 Rectification ratio statistical diagram of chiral nanochannels with amino acid molecules in different conformations added to D-phenylalanine-modified gold nanomembranes, Figure 5 Statistical diagram of the rectification ratio of chiral nanochannels with amino acid molecules in different conformations added to L-phenylalanine-modified gold nan...

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Abstract

A method for recognizing amino acid enantiomers by adopting a chiral gold nano-channel belongs to the technical field of ion channel construction and chiral recognition. According to the invention, achiral nano-channel is placed in the middle of an electrolyte sample pool with holes to be used as an ion channel, and the recognition of different amino acid enantiomers can be realized by adopting an electrochemical test on ion transmission signals of amino acids containing different enantiomers. By using the method, recognition of dozens of amino acid configurations can be realized. Among currently known amino acids, the enantiomer recognition sensitivity of tryptophan is the highest. According to the method, the chiral configuration is recognized through ion transmission, and huge possibility is provided for establishment of a new chiral detection method and application expansion of an ion channel.

Description

technical field [0001] The invention relates to a method for identifying amino acid enantiomers by using a chiral gold nanochannel, and belongs to the technical field of ion channel construction and chiral recognition. Background technique [0002] Ion channels, as an integral part of regulating the normal life activities of living organisms, play an important role in material recognition, energy conversion, and body activities. According to relevant reports, the natural ion channels in the body are chiral structural proteins. Therefore, the study of the material recognition mechanism of chiral nanochannels has always been a research hotspot. However, the natural ion channel is a protein, and the protein's volatile and fragile structure limits the study of ion channels to a certain extent. [0003] The development of precise artificial nanomanipulation technology provides a new idea for the construction of artificial nanochannels. The controllable size of nanochannels and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416
CPCG01N27/416
Inventor 胥传来蔡佳蓉匡华徐丽广马伟孙茂忠刘丽强吴晓玲宋珊珊胡拥明
Owner JIANGNAN UNIV
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