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Method for biologically removing tetracycline antibiotics

A tetracycline and antibiotic technology, applied in the field of environmental remediation, can solve problems such as tetracycline antibiotics that have not been removed, and achieve the effects of improving biological removal efficiency, reducing biological toxicity, and eliminating separation and purification.

Active Publication Date: 2020-03-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the biological removal of tetracycline antibiotics by the mixed fermentation of Bacillus clausii and bacteria that can produce surfactants

Method used

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  • Method for biologically removing tetracycline antibiotics
  • Method for biologically removing tetracycline antibiotics
  • Method for biologically removing tetracycline antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The first seed medium, tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, the balance is water.

[0032] The second seed medium, glucose 60g / L, beef extract 10g / L, peptone 10g / L, yeast extract 10g / L, NaCl 5g / L, and the balance is water.

Embodiment 2

[0034] The formula of the fermentation medium is: glucose 55g / L; yeast extract powder 8g / L; peptone 8g / L; NaCl 8g / L; KCl 0.8g / L; K 2 HPO 4 5 g / L; KH 2 PO 4 5 g / L; MgSO 4 ·7H 2 O 0.8g / L; FeSO 4 ·7H 2 O 1.5×10 -3 g / L; CuSO 4 ·5H 2 O 1.5×10 -5 g / L; MnSO 4 8×10 -5 g / L; ZnSO 4 ·7H 2 O 1.5×10 -3 g / L; the rest is water.

Embodiment 3

[0036] The formula of the fermentation medium is: glucose 65g / L; yeast extract powder 3g / L; peptone 3g / L; NaCl 3g / L; KCl 0.3g / L; 2 HPO 4 1 g / L; KH 2 PO 4 1 g / L; MgSO 4 ·7H 2 O 0.3g / L; FeSO 4 ·7H 2 O 0.5×10 -3 g / L; CuSO 4 ·5H 2 O 0.5×10 -5 g / L; MnSO 4 5×10 -5 g / L; ZnSO 4 ·7H 2 O 0.5×10 -3 g / L; the rest is water.

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Abstract

The invention discloses a method for biologically removing tetracycline antibiotics. The method comprises the following steps: (1) seed culture: culturing secondary seeds of bacillus clausii and bacillus amyloliquefaciens; and (2) removal: mixing a water body containing tetracycline antibiotics with a fermentation medium to prepare a degradation solution, inoculating bacillus clausii secondary seeds and HM-618 secondary seeds into the degradation solution, and fermenting. A co-culture system of bacillus clausii and bacillus amyloliquefaciens is established and added into a water body containing tetracycline antibiotics, so that the bacillus amyloliquefaciens produces surface active substances in situ to promote biological removal of the tetracycline antibiotics, and a series of tedious steps such as separation and purification of a surfactant are omitted; according to the method, the biological removal efficiency of the tetracycline antibiotics is improved, and the biotoxicity of tetracycline antibiotic conversion products is remarkably reduced.

Description

technical field [0001] The invention belongs to the field of environmental restoration and relates to a method for biologically removing tetracycline antibiotics assisted by surfactants. Background technique [0002] Tetracycline antibiotics represented by oxytetracycline (OTC) and chlorotetracycline (CTC) are a class of broad-spectrum antibiotics, widely used in animal husbandry and aquaculture, and their usage accounts for 14% of the total antibiotic usage. Tetracycline antibiotics were detected in all types of water environment. It not only leads to serious water pollution, threatens the survival of aquatic plants, animals and microorganisms, but also leads to the emergence of tetracycline resistance in bacteria and the spread of resistance genes. Therefore, it is urgent to find effective ways to remove tetracycline antibiotics from water . [0003] Traditional physical methods such as adsorption and photocatalysis are costly and easily cause secondary pollution. Many ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F101/38
CPCC12N1/20C02F3/341C02F2101/38
Inventor 程景胜刘晓春元英进
Owner TIANJIN UNIV
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