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Preparation and detection method of escherichia coli electrochemical biosensor

A technology of biosensor and Escherichia coli, which is applied in the field of biomedicine, can solve the problems of poor electrode stability, weak selectivity, and poor stability of electrochemical sensors, and achieve the effects of good stability, simple preparation, and strong selectivity

Active Publication Date: 2020-03-24
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional bioelectrochemical sensors often have problems such as poor electrode stability and weak selectivity. Improving the selectivity, stability, and sensitivity of bioelectrochemical sensors has become the focus of research.
In the past research, it was found that the dual-electron mediator system can amplify the chemical signal by more than 5 orders of magnitude and improve the detection sensitivity; however, the stability of the original electrochemical sensor is poor. Therefore, we further improved the electrode preparation method and achieved high stability to E. coli , high-sensitivity detection

Method used

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  • Preparation and detection method of escherichia coli electrochemical biosensor
  • Preparation and detection method of escherichia coli electrochemical biosensor
  • Preparation and detection method of escherichia coli electrochemical biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053]Embodiment 1: step 1, the glassy carbon electrode, that is, the GCE electrode is placed in the chitosan solution, that is, the Chi solution, and the pH value of the Chi solution is adjusted to 6 with NaOH solution, and Chi is deposited on the GCE electrode by cyclic voltammetry; Washing and drying to obtain Chi-GCE electrodes;

[0054] Step 2, soak the Chi-GCE electrode in the dopamine solution, that is, the DA solution, and use a three-electrode system and cyclic voltammetry to electropolymerize DA to prepare a dopamine-chitosan-GCE modified electrode, that is, DA-Chi- GCE modified electrode, cleaned and dried;

[0055] Step 3, soak the DA-Chi-GCE modified electrode in D-mannose solution, that is, D-man solution, and use PBS solution concentration of 0.1 M / L to prepare D-mannose-dopamine-chitosan -GCE modified electrode, that is, D-man-DA-Chi-GCE modified electrode, forming a D-man-DA-Chi sensor;

[0056] Step 4, the D-man-DA-Chi-GCE modified electrode is placed in th...

Embodiment 2

[0081] Embodiment 2: step 1, place the glassy carbon electrode, i.e. the GCE electrode in the chitosan solution, i.e. the Chi solution, adjust the pH value of the Chi solution to 6 with NaOH solution, and deposit Chi to the GCE electrode by cyclic voltammetry; Washing and drying to obtain Chi-GCE electrodes;

[0082] Step 2, soak the Chi-GCE electrode in the dopamine solution, that is, the DA solution, and use a three-electrode system and cyclic voltammetry to electropolymerize DA to prepare a dopamine-chitosan-GCE modified electrode, that is, DA-Chi- GCE modified electrode, cleaned and dried;

[0083] Step 3, soak the DA-Chi-GCE modified electrode in D-mannose solution, that is, D-man solution, and use PBS solution concentration of 0.08 M / L to prepare D-mannose-dopamine-chitosan -GCE modified electrode, that is, D-man-DA-Chi-GCE modified electrode, forming a D-man-DA-Chi sensor;

[0084]Step 4, the D-man-DA-Chi-GCE modified electrode is placed in the double electron mediato...

Embodiment 3

[0085] Embodiment 3: Step 2 and Step 3 are interchanged to form Step N2 and Step N3:

[0086] Step 1, the glassy carbon electrode, that is, the GCE electrode is placed in the chitosan solution, that is, the Chi solution, the pH of the Chi solution is adjusted to 5 with NaOH solution, and Chi is deposited on the GCE electrode by cyclic voltammetry; cleaning and drying are obtained Chi-GCE electrodes;

[0087] Step N2, immerse the Chi-GCE electrode in D-mannose solution, that is, D-man solution, and use a PBS solution concentration of 0.15 M / L to prepare a D-man-Chi-GCE modified electrode, and wash ,dry;

[0088] Step N3, soaking the D-man-Chi-GCE modified electrode in a dopamine solution, that is, a DA solution, using a three-electrode system and cyclic voltammetry to electropolymerize DA to prepare dopamine-D-mannose-chitosan -GCE modified electrode, that is, DA-D-man-Chi-GCE modified electrode to form a DA-D-man-Chi sensor;

[0089] Step 4, the D-man-DA-Chi-GCE modified el...

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PUM

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Abstract

The invention relates to a preparation and detection method of an electrochemical biosensor for escherichia coli, belonging to the field of biomedicine. The invention provides a preparation method andapplication of a D-mannose-dopamine-chitosan modified electrochemical biosensor based on Fc-Ru < 3 + > mediation of a double-electron mediator . A sensor comprises an electrode material, surface filmmodification of an electrode, an immobilized bioactive compound and the double-electron mediator, and the electrode material is glassy carbon. The electrode surface film is a dopamine and chitosan film; wherein an immobilized bioactive compound is D-mannose; and a component of the dual-electron mediator is Fc-Ru < 3 + >. The electrochemical sensor has the advantages of high response sensitivity to escherichia coli in a detection system, strong selectivity, good stability and simple preparation, and can be used for escherichia coli detection.

Description

technical field [0001] The invention relates to a preparation and detection method of an Escherichia coli electrochemical biosensor, belonging to the field of biomedicine. Background technique [0002] Escherichia coli (E.coli) is one of the food-borne pathogens. With the globalization of food trade, diseases caused by it appear frequently. Infection with Escherichia coli can cause cholecystitis, bacteremia, cholangitis and diarrheal diseases. Traditional food detection methods mainly include plate culture method and paper disk method. The detection time takes 2-3 days and cannot be monitored in real time. Therefore, it is necessary to find a fast and sensitive detection method for E. coli detection in health care, environmental testing, food analysis and safety. An urgent need in the field of control. [0003] Electrochemical biosensors are fast, simple, and inexpensive analytical devices that promise to be effective tools for bacterial detection. It consists of four part...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/308G01N27/327G01N27/48
Inventor 刘正春来庆腾陈智睿姚晓霞陈伟丁家峰梁波
Owner CENT SOUTH UNIV
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