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Ectoine-producing yeast

A technology of ectoine and yeast, applied in the field of biological production of ectoine, can solve the problems of complicated process, increased cost, high salt concentration, etc.

Active Publication Date: 2020-03-24
GIVAUDAN SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods require high salt concentration, which complicates the process and leads to increased costs involved considering significant corrosion of equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

no. 1 approach

[0657] According to a first embodiment, the inducible or repressible promoter according to the invention may be selected from the group comprising: promoter inducible or repressible with copper, promoter inducible or repressible with methionine promoters and promoters inducible or repressible with threonine, and are particularly selected from the group consisting of:

[0658] pSAM4-methionine inducible or repressible (SEQ ID NO. 38),

[0659] pCUP1-1 - copper inducible or repressible (SEQ ID NO. 39),

[0660] pCUP1.cgla - copper inducible or repressible (SEQ ID NO. 40),

[0661] pCUP1.sba - copper inducible or repressible (SEQ ID NO. 41),

[0662] pACU1 - copper inducible or repressible (SEQ ID NO. 42),

[0663] pACU2 - copper inducible or repressible (SEQ ID NO. 43),

[0664] pACU3p - copper inducible or repressible (SEQ ID NO. 44),

[0665] pACU4p - copper inducible or repressible (SEQ ID NO. 45),

[0666] pACU5 - copper inducible or repressible (SE...

no. 2 approach

[0682] According to a second embodiment, the inducible or repressible promoter according to the invention may be selected from the group comprising: promoters inducible or repressible with copper, promoters inducible or repressible with lysine and a promoter inducible or repressible with methionine, and is particularly selected from the group consisting of:

[0683] pCTR1 - copper inducible or repressible (SEQ ID NO. 60),

[0684] pCTR3 - copper inducible or repressible (SEQ ID NO. 61),

[0685] pCUR1 - copper inducible or repressible (SEQ ID NO. 62),

[0686] pCUR2 - copper inducible or repressible (SEQ ID NO. 63),

[0687] pCUR3 - copper inducible or repressible (SEQ ID NO. 64),

[0688] pCUR4 - copper inducible or repressible (SEQ ID NO. 65),

[0689] pCUR5p - copper inducible or repressible (SEQ ID NO. 66),

[0690] pCUR6 - copper inducible or repressible (SEQ ID NO. 67),

[0691] pCUR7 - copper inducible or repressible (SEQ ID NO. 68),

[0692]...

Embodiment 1

[0796] Example 1: Protocol for the preparation of recombinant Saccharomyces cerevisiae strains according to the invention

[0797] All recombinant S. cerevisiae strains performed below were constructed from standard strains using standard yeast molecular genetics procedures (D. Burke, D. Dawson's Methods in yeast Genetics - A cold spring harbor laboratory course Manual (2000), T. Stearns CSHL Press). made.

[0798] Taking advantage of the ability of yeast to efficiently recombine free DNA ends with sequence homology, clusters of the following genes were integrated in recombinant yeast at once.

[0799] Additionally, to better understand the following genotypes:

[0800] -ade2, his3, leu2, trp1 and ura3 are auxotrophic marker genes.

[0801] - Lower case letters mean that the considered gene is inactive, upper case letters indicate active genes.

[0802] - "::": after the gene name, meaning that the gene is interrupted by the following material (if more than...

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PUM

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Abstract

The present invention relates to the field of bio-production of ectoine. There is a need in the art for ectoine production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a genetically modified yeast comprising many modifications as described in the present text.

Description

technical field [0001] The present invention relates to the field of biological production of ectoine. Background technique [0002] Ecdoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) is a heterocyclic amino acid naturally produced by halophilic organisms in nature. Indeed, in order to survive in a saline environment, these organisms produce ectoine as a compatible solute, which serves as an osmotic balance. [0003] Ectoine can indeed protect nucleic acid, protein, cell membrane and the whole cell from denaturation caused by a large amount of damage from the external environment (such as ultraviolet radiation, heating, freezing or chemical agents), and also from denaturation caused by drying (See eg Lentzen G et al. Appl Microbiol Biot. 2006; 72:623-34, and Graf R et al. Clin Dermatol. 2008; 26:326-33). Therefore, it is used in cosmetics for skin care. [0004] Furthermore, ectoine has been found to be interesting as a protein stabilizer, cosmetic additive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/81C12P17/12
CPCC12N15/815C12P17/12C12Y102/01011C12Y207/02004C12Y203/01031C12Y402/01108C12Y203/01178C12Y206/01076C12N15/81C12Y104/01002C12N9/0006C12N9/0008C12N9/1029C12N9/1096C12N9/1217C12N9/80C12N9/88C12Y101/01003
Inventor 多米尼克·路易斯卡琳·杰尔拉尔登多米尼克·托马斯
Owner GIVAUDAN SA
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