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Cultivation method of cordyceps militaris with stable and high-content cordycepic acid

A cultivation method, the technology of Cordyceps militaris, which is applied in the field of fungal sporocarp production, can solve the problems of high content of cordycepic acid, instability, and few results, and achieve the effects of simple operation, stable content, and easy storage

Inactive Publication Date: 2020-03-27
SHANXI WANHAIAO BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the artificial cultivation of Cordyceps militaris has become an emerging industry. Many researchers have carried out detailed research on the active substances of Cordyceps militaris, and the research on cordycepic acid has also been listed as an important topic, but there are not many results.
Chinese patent CN103981105.A discloses that the cordycepic acid content range of Cordyceps militaris is 18-25%, but high and unstable is its unsatisfactory point
[0004] In order to solve the defect of high and unstable content of Cordyceps militaris in the existing artificial cultivation of Cordyceps militaris, the present invention has developed a cultivation method of fruiting body of Cordyceps militaris with stable and high content of Cordyceps militaris

Method used

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  • Cultivation method of cordyceps militaris with stable and high-content cordycepic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 (Applying Cordyceps militaris anti-degeneration strain bottle cultivation)

[0027] A method for cultivating cordyceps militaris with stable high content of cordycepin acid comprises the steps:

[0028] 1) Preparation of Cordyceps militaris compound liquid species:

[0029] a: liquid seed formula: peeled potato 250g, earthworm 10g, ginkgo fruit 10g, sucrose 25g, potassium dihydrogen phosphate 1.2g, dipotassium hydrogen phosphate 1.8g, magnesium sulfate 1.5g, vitamin B 1 15mg, vitamin B 6 10mg, water 1000ml;

[0030] b: preparation method: grind the ground dragon powder, cut the ginkgo fruit into pieces, then mix and cook for 40 minutes, collect the filtrate; cook the potato pieces in the filtrate for 20 minutes, collect the secondary filtrate; make up the water to 1000m1, add other components Boil and dissolve, pack in 500m1 conical bottles, each bottle is 120ml, sealed with cotton plug polypropylene, sterilized at high temperature of 120-121℃ for 30 minute...

Embodiment 2

[0038] Embodiment 2 (application of Cordyceps militaris anti-degeneration strain special box cultivation)

[0039] A method for cultivating cordyceps militaris with stable high content of cordycepin acid comprises the steps:

[0040] 1) Preparation of Cordyceps militaris compound liquid species:

[0041] a: liquid seed formula: take the following ingredients by weight: peeled potato 200, earth dragon 10, ginkgo fruit 10, sucrose 25, potassium dihydrogen phosphate 1.2, dipotassium hydrogen phosphate 1.8, magnesium sulfate 1.5, vitamin B 1 0.015, vitamin B 6 0.01, water 1000;

[0042] b: preparation method: the above-mentioned earth dragon milled powder, ginkgo fruit are cut into pieces, mixed and cooked for 40 minutes, and the filtrate is collected; the potato is cut into small pieces and cooked in the filtrate for 20 minutes, and the secondary filtrate is collected; make up water to 1000, add other components Boil and dissolve, divide into 500m1 culture bottles, each bottle...

Embodiment 3

[0049] Example 3 (Applying simple canning bottle cultivation, domestication of commercially available strains into anti-degeneration strains)

[0050] A method for cultivating cordyceps militaris with stable high content of cordycepin acid comprises the steps:

[0051] 1) Preparation of Cordyceps militaris composite liquid species: 2 parent species of Cordyceps militaris were purchased from Yichun Friendly Edible Fungi Research Institute, Heilongjiang, and cultivated into anti-degeneration parent species according to the method disclosed in Patent No. ZL201210248399.6;

[0052] a: liquid seed formula: take the following ingredients by weight: peeled potato 200, earth dragon 10, ginkgo fruit 10, sucrose 25, potassium dihydrogen phosphate 1.2, dipotassium hydrogen phosphate 1.8, magnesium sulfate 1.5, vitamin B 1 0.015, vitamin B 6 0.01, water 1000;

[0053] b: preparation method: the above-mentioned earthworm milled powder, ginkgo fruit are chopped, mixed and boiled for 40 mi...

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Abstract

The invention discloses a cultivation method of cordyceps militaris with stable high-content cordycepic acid, belongs to the technical field of fungal sporocarp production, and relates to cordyceps militaris. The method comprises the following steps: 1) preparing a cordyceps militaris composite liquid strain: inoculating a cordyceps militaris mother strain with a monascus strain according to a ratio of 1: 1 on the same liquid culture medium, and carrying out co-fermentation culture to obtain the liquid strain; 2) preparing a Chinese herbal medicine growth culture medium: sub-packaging 60-80% of unhulled rice and 20-40% of earthworm into 500-6000ml of cultivation containers, and then adding a nutrient solution of which the volume is 1.2-1.4 times that of the growth culture medium; 3) inoculating, culturing, harvesting and processing cordyceps militaris; the method is easy to operate and popularize, the content of cordycepic acid in the cordyceps militaris can be remarkably increased, the content of the cordycepic acid is stable, and a finished product is easy to store.

Description

technical field [0001] The invention belongs to the technical field of fungal fruiting body production, relates to Cordyceps militaris, and in particular relates to a method for cultivating Cordyceps militaris with stable and high content of cordycepic acid. Background technique [0002] Cordycepic acid, also known as D-mannitol, is one of the important active substances of Cordyceps militaris. It has the functions of preventing and treating cerebral thrombosis, cerebral hemorrhage, renal failure and diuresis. It belongs to the list of important medicines of the World Health Organization. very important essential medicines. Cordycepin is commonly found in many plant fungi, but the highest content in Cordyceps militaris is generally between 1.97-7.00%. [0003] In recent years, artificial cultivation of Cordyceps militaris has become an emerging industry. Many researchers have carried out detailed research on the active substances of Cordyceps militaris, and research on Cord...

Claims

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Application Information

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IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 杨毅周文贵周翔肖文泽
Owner SHANXI WANHAIAO BIOLOGICAL TECH CO LTD