A kind of dairy cow production performance measurement standard substance and its preparation method and application

A technology of production performance and reference material, applied in biological testing, other equipment, applications, etc., can solve the problems of increased confidence interval of calibration linear regression curve, narrow component concentration range, high leverage value, and achieve increased consistency and measurement level. Improved, more accurate effects

Active Publication Date: 2022-03-04
全国畜牧总站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This standard material is characterized by a narrow range of component concentrations, a relatively high leverage value, and a positive correlation between fat and protein concentrations. These factors will affect the accuracy of the standard material, usually as an increase in the confidence interval of the calibration linear regression curve. Large, and its shelf life at 4°C is short, 15 days

Method used

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  • A kind of dairy cow production performance measurement standard substance and its preparation method and application
  • A kind of dairy cow production performance measurement standard substance and its preparation method and application
  • A kind of dairy cow production performance measurement standard substance and its preparation method and application

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Effect test

Embodiment 1

[0055] The design of embodiment 1DHI standard substance

[0056] The present embodiment provides a kind of dairy cow production performance determination (DHI) standard substance, comprising:

[0057] The mass percent range of fat is 0.20%-6.00%, and 12 level gradients are designed; the mass percent range of protein is 2.00%-4.50%, and 6 level gradients are designed; the mass percent range of lactose is 4.00%-5.00%, and 4 levels are designed A horizontal gradient, according to the composition of the above-mentioned mass percentage range and the corresponding horizontal gradient, DHI standard substance is obtained through orthogonal design, and the DHI standard substance includes standard substance 1-12, as shown in Table 1 below:

[0058] Table 1 DHI standard substance

[0059]

Embodiment 2

[0060] The deployment of embodiment 2 DHI standard substance

[0061] 1. Deployment method

[0062] The raw materials (cream, skimmed milk, membrane retentate and membrane filtrate) obtained by the pre-experiment were determined by FOSS milk component analyzer and chemical determination method to determine the content of fat, protein and lactose respectively. In addition, add pure water and analytical pure lactose as raw materials, input into the EXCEL form; use its planning and solving function to calculate the amount of raw materials for each standard product, and then carry out formula deployment; determine the chemical composition of the product by chemical determination, and investigate its difference with the standard product degree of compliance. Table 2 below is an example of the preparation of Standard 1.

[0063] Table 2 Standard product 1 deployment table

[0064]

[0065]

[0066] 2. Inspection of the prepared DHI standard substance

[0067] Send the prod...

Embodiment 3

[0079] The preparation of embodiment 3 DHI standard substance

[0080] The present embodiment provides a kind of preparation method for preparing DHI standard substance, comprises the following steps:

[0081] (1) Sterilize the tubular sterilizer with hot water above 95°C, then take raw milk and add it to the tubular sterilizer for heat treatment, the heat treatment temperature is 50°C, and the treatment time is 18s;

[0082] (2) The heat-treated raw milk is directly added to a milk fat separator for skimming treatment, and the obtained skimmed milk and milk fat are respectively put into different storage tanks for subsequent use;

[0083](3) Carry out microfiltration sterilization to skimmed milk, filter equipment adopts PALL ceramic membrane filtration equipment, filter membrane selects the ceramic membrane column of 1.4 micron order, described ceramic membrane column is installed to PALL ceramic membrane filtration equipment, with not more than 8 Heat up at a rate of ℃ / min...

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Abstract

The present invention provides a dairy cow production performance measurement standard substance and its preparation method and application, which comprises the components and corresponding horizontal gradients obtained by orthogonal design according to the following mass percentage range: the mass percentage range of fat is 0.20%-6.00%, Design 12 levels of gradients; the mass percent range of protein is 2.00%-4.50%, and 6 levels of gradients are designed; the mass percent range of lactose is 4.00%-5.00%, and 4 levels of gradients are designed; Uniform and stable, it meets the requirements of DHI determination work, the concentration range of components is wide, and the leverage value is relatively low, which reduces the influence of the correlation between fat and protein content in natural milk samples on the calibration accuracy of DHI standard substances. Using the DHI After the instrument is calibrated by the standard material, the accuracy of the instrument is improved, the determination level is improved and remains stable, and the consistency between laboratories is increased.

Description

technical field [0001] The invention belongs to the field of dairy cow production performance measurement, and in particular relates to a dairy cow production performance measurement standard substance and its preparation method and application. Background technique [0002] Dairy Herd Improvement (DHI), also known as dairy herd improvement, aims to measure the lactation performance and milk components of dairy cows and establish a complete record system. It is also the core and basis of digital breeding technology for dairy cows. DHI measurement technology was born and applied in 1906. After more than 100 years of development, it has become an important means of "cultivating fine varieties, implementing scientific breeding and improving raw milk quality". DHI is a comprehensive technology commonly used by developed countries in the world's dairy industry to manage and improve the production level of dairy cows. It is recognized by the industry as "the only effective method ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K29/00G01N21/3577G01N33/68
CPCA01K29/005G01N21/3577G01N33/68
Inventor 李丽丽刘婷婷李竞前马学升白文娟陈建坡李姣韩旭赵俊金何丽孟飞刘刚朱芳贤许海涛冯海永隋鹤鸣
Owner 全国畜牧总站
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