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Method for determining glipizide concentration in blood plasma by liquid chromatography-mass spectrometry

A technique for glipizide and LC/MS, applied in the field of medicine, can solve problems such as speed, precision and sensitivity to be improved, and achieve the effects of simple pretreatment method, simple operation and high sensitivity

Inactive Publication Date: 2020-03-27
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the speed, precision and sensitivity of the existing methods for the determination of glipizide need to be improved

Method used

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  • Method for determining glipizide concentration in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining glipizide concentration in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining glipizide concentration in blood plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Glipizide Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment

[0034] Glipizide (analyte): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0035] Glipizide-d11 (internal standard): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0036] The reagents used are shown in Table 1 below:

[0037] Table 1 Reagent Details

[0038] Reagent name level manufacturer Acetonitrile HPLC J. T. Baker Methanol HPLC J. T. Baker formic acid ACS Adamas

[0039] Note: Reagents of the same grade or higher can also be used

[0040] The analytical equipment used is shown in Table 2 below:

[0041] Table 2 Details of equipment used

[0042]

[0043]

[0044] The same LC / MS / MS system can also be used.

[0045] 2. Liquid conditions

[0046] 1. Liquid chromatography conditions

[0047] Chromatographic column: Agilent ZORBAX...

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Abstract

The invention discloses a method for determining glipizide concentration in blood plasma by liquid chromatography-mass spectrometry, which adopts a liquid chromatography-mass spectrometry system for determination, and comprises the following steps: taking a sample to be determined, adding a certain amount of mixed organic solvent for extraction and pretreatment, performing separating by using a chromatographic column, and performing detecting by using a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of glipizide; the linear range of a plasma standard curve of the method is 1-500 ng / mL, the intra-batch precision RSD and the inter-batch precision RSD are both smaller than + / -15%, and the method is suitable for measuring the concentration of glipizide in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for measuring medicines, in particular to a method for measuring the concentration of glipizide in blood plasma by liquid mass spectrometry. Background technique [0002] Due to the improvement of living standards and the aggravation of aging, the number of diabetic patients is on the rise year by year; according to incomplete statistics, the incidence of diabetes in all countries in the world is on the rise, and type II diabetes patients account for more than 90%. In recent years, the rising rate of diabetes in developed countries is 45%, and the rising rate in developing countries is as high as 200%. More and more data show that diabetes is very common in China, India, and some developing countries in Africa. common and popular. In recent years, the increase trend of diabetes patients in my country has been on the rise. According to statistics in 2003, there were ab...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/72
CPCG01N30/06G01N30/34G01N30/72G01N30/88G01N2030/045G01N2030/062G01N2030/8822G01N2030/884
Inventor 林明弘
Owner 徐州立兴佳正医药科技有限公司