Duplex test strip for detecting methamphetamine and ketamine as well as preparation method and application method of duplex test strip

Abstract

The invention relates to a duplex test strip for detecting methamphetamine and ketamine as well as a preparation method and an application method of the duplex test strip. The test strip sequentiallycomprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody and a ketamine antibody marked by time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a ketamine antigenand a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of methamphetamine and ketamine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting the methamphetamine and ketamine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

Description

technical field [0001] The invention relates to the detection of methamphetamine and ketamine, in particular to a dual test strip for simultaneous quantitative detection of methamphetamine and ketamine by using time-resolved fluorescent microsphere immunochromatography technology, and a preparation method and an application method thereof. Background technique [0002] Methamphetamine, commonly known as "ice", is a stimulant psychotropic drug with a strong central nervous system excitatory effect. It is a class of psychotropic drugs and narcotic drugs strictly controlled by the state. At present, the abuse of amphetamine-like substances in the world is on the rise. Experts predict that amphetamine-type stimulants will become one of the most widely abused drugs of the 21st century. [0003] As criminal cases related to methamphetamine increase year by year and the country's management of methamphetamine becomes increasingly strict, this field puts forward higher requirements...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of existing methods for detecting methamphetamine and ketamine, which are complex in operation, time-consuming, unable to realize rapid detection of large batches of samples, and unable to simultaneously detect methamphetamine and ketamine, Provide a test strip for detecting methamphetamine and ketamine with simple operation, high sensitivity, fast detection speed and low cost, its preparation method and application method, so as to realize rapid detection of methamphetamine and ketamine simultaneously on a large number of samples and on-site monitoring

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