A kind of fungal agent for promoting the growth of Arvensis scalloped container seedlings and its preparation method and application
The technology of C. serratus and container seedlings is applied in the field of inoculum for promoting the growth of C. sylvatica container seedlings and the field of preparation thereof. Ability to external stress, increase in plant height, increase in biomass
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[0023] The present invention also provides a preparation method for the bacteria agent described in the above scheme: inoculate the Glomus ciliatus strain and the Glomus septum marcescens strain on the sterilized propagation substrate, plant corn and / or clover, and make the The strains of Glomus mycelium and Glomus septaceta were fully propagated between the propagation substrate and the plant root system, and the final harvest consisted of the propagation substrate dry soil, root hyphae, root hyphae and infected root segments Inoculum agent of Glomus aureus strain and Glomus septae strain; the effective number of spores of the inoculum agent is preferably 300~480 / 60g.
[0024] Wherein, the present invention has no special requirements on the propagation substrate, and the conventional propagation substrate in the field can be used. In the specific implementation process of the present invention, the propagation substrate is composed of river sand and garden soil in a mass rati...
Embodiment 1
[0035] 1. Choose garden soil: peat: river sand = 3:1:1 according to the volume ratio, pass through a 2mm sieve, mix evenly, sterilize with high temperature (121°C) high pressure (0.1MPa) steam for 0.5h, and cool naturally to obtain the matrix. The basic physical and chemical properties of the matrix are: pH value 5.99, organic matter content 40.458g·kg -1 ;Organic carbon: 69.750g·kg -1 ;Total phosphorus: 0.754g·kg -1 ;Total nitrogen: 1.903g·kg -1 ; Ammonium nitrogen: 15.068mg·kg -1 ;Nitrate nitrogen: 4.209mg·kg -1 .
[0036] 2. Select the full-bodied Jacuzzi scutellaria seeds collected in the same year, and use 10% H 2 o 2 The solution was soaked and disinfected for 10 minutes, and then rinsed with sterile water 2 o 2 The residual liquid is soaked in warm boiled water cooled to 65°C. After it is naturally cooled to room temperature (25°C), it is continuously soaked in sterile water for 5 days at room temperature (change the water once a day). Then put it into a 25°C i...
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