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Method for preparing anti-bacteria composition, prepared anti-bacteria composition and application of anti-bacteria composition

A composition and mixture technology, applied in the field of medicine, can solve problems such as antibiotic resistance

Active Publication Date: 2020-04-07
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antibiotics are mainly used at home and abroad to inhibit Staphylococcus aureus, but Staphylococcus aureus is prone to develop resistance to antibiotics

Method used

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  • Method for preparing anti-bacteria composition, prepared anti-bacteria composition and application of anti-bacteria composition
  • Method for preparing anti-bacteria composition, prepared anti-bacteria composition and application of anti-bacteria composition
  • Method for preparing anti-bacteria composition, prepared anti-bacteria composition and application of anti-bacteria composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1 Preparation of Eucommia Leaf Extract

[0088] Take 50 g of the chopped Eucommia leaves, add 8 times the weight of water, reflux and extract at 100°C for 1.5 hours, filter to obtain filtrate and filter residue, repeat the above extraction operation on the filter residue, and combine the obtained filtrate twice. The filtrate was rotary evaporated at 60°C into a thick extract, and the thick extract was vacuum dried at 60°C to obtain a dry paste. The yield of the dry paste was 33% (W / W).

[0089] Weigh an appropriate amount of dry ointment according to the dry ointment yield, add sterilized water to prepare drug stock solutions with crude drug concentrations of 1000mg / mL, 500mg / mL, 100mg / mL, and 50mg / mL, and store them at 4°C. Under aseptic conditions, mix 1 mL of the drug stock solution with 9 mL of MRS medium and pour it into a sterile plate immediately. After cooling, the concentration of crude drug is 100mg / mL, 50mg / mL, 10mg / mL, 5mg / mL, respectively. Of eucommia l...

Embodiment 2

[0090] Example 2 Preparation of Lactobacillus bulgaricus strain and determination of viable bacterial count

[0091] (1) Preparation of Lactobacillus bulgaricus strain:

[0092] The activated Lactobacillus bulgaricus strain is transferred to the MRS liquid medium, and statically cultured at 37° C. for 16-18 hours to obtain the Lactobacillus bulgaricus strain.

[0093] (2) Determination of the number of viable bacteria in Lactobacillus bulgaricus strain:

[0094] Use sterilized water to perform 10 5 Double dilution to obtain diluent. Spread 50 μL of the dilution solution on the MRS solid medium plate, culture at 37° C. for 24 hours at a constant temperature, count the plates with 30-300 colonies, and perform the parallel measurement 3 times to calculate the average number of colonies on the plate. Then, calculate the number of viable bacteria per unit volume of Lactobacillus bulgaricus seed liquid according to the following formula.

[0095] Z=(C / V)×M

[0096] among them:

[0097] Z rep...

Embodiment 3

[0102] Example 3 Preparation of antibacterial composition 1

[0103] Add 25μL of MRS liquid medium and 3μL of crude drug to 50mg·mL of Lactobacillus bulgaricus strain prepared in Example 2 -1 Eucommia ulmoides leaf extract (prepared in Example 1), gently stirred with a pipette to obtain a mixture; the number of viable Lactobacillus bulgaricus in the mixture was 4.8×10 6 CFU / mL, the crude drug concentration of Eucommia ulmoides leaves is 5mg·mL -1 ; The mixture was incubated at 37° C. for 24 hours at a constant temperature to obtain antibacterial composition 1.

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Abstract

The invention belongs to the field of medicines, in particular to a method for preparing an anti-bacteria composition. The method comprises the following steps of: (1) mixing a lactobacillus bulgaricus culture with folium eucommiae extracts to obtain a mixture, wherein the viable count of the lactobacillus bulgaricus in the mixture is 4.0*10<6>-7.0*10<6> CFU / mL, and the crude drug concentration ofthe folium eucommiae in the mixture is 1-9.6 mg / mL; and (2) culturing the mixture obtained in S(1) at a temperature of 20-38 DEG C for 20-30 hours to obtain the anti-bacteria composition. The invention also relates to the prepared anti-bacteria composition. The anti-bacteria composition prepared with the method has an obvious anti-bacteria effect.

Description

Technical field [0001] The invention belongs to the field of medicine, and specifically relates to a method for preparing a bacteriostatic composition, as well as the prepared bacteriostatic composition and its application. Background technique [0002] Staphylococcus aureus is widely distributed in nature and easily infects animals and humans. At present, antibiotics are mainly used at home and abroad to inhibit Staphylococcus aureus, but Staphylococcus aureus is easily resistant to antibiotics. Moreover, broad-spectrum antibiotics have a significant impact on the intestinal flora, easily disrupting the balance of the intestinal microecosystem and causing a series of related diseases, such as gastrointestinal diseases, inflammatory diseases, rheumatoid arthritis, multiple sclerosis, Diabetes, metabolic syndrome, obesity, etc. [0003] Based on this situation, there is a need for a drug that inhibits bacteria and does not disrupt the balance of the intestinal microecosystem. Pro...

Claims

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Application Information

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IPC IPC(8): A61K36/46A61P31/04A01N65/08A01N63/00A01P1/00A61K35/747
CPCA61K36/46A61K35/747A61P31/04A01N65/00A01N65/08A01N63/00A61K2236/19A61K2300/00
Inventor 赵鑫高秀梅王亚静刘二伟石惠姝
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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