Blue-green haworthia succulent plant (laworthia succulent plant lanjing) germplasm resource in-vitro preservation method

A technology for in vitro preservation and succulent plants, which is applied in the field of in vitro preservation of the germplasm resources of the succulent plant succulents of the succulent plant, can solve the problems of damage to succulent plants and high storage costs in liquid nitrogen, and reduce the cost and difficulty of preservation. Convenient transportation and storage, simple operation

A technology for in vitro preservation and succulent plants, which is applied in the field of in vitro preservation of the germplasm resources of the succulent plant succulents of the succulent plant, can solve the problems of damage to succulent plants and high storage costs in liquid nitrogen, and reduce the cost and difficulty of preservation. Convenient transportation and storage, simple operation

CN110972936AActive Publication Date: 2020-04-10绍兴市农业科学研究院
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  • Blue-green haworthia succulent plant (laworthia succulent plant lanjing) germplasm resource in-vitro preservation method
  • Blue-green haworthia succulent plant (laworthia succulent plant lanjing) germplasm resource in-vitro preservation method
  • Blue-green haworthia succulent plant (laworthia succulent plant lanjing) germplasm resource in-vitro preservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The blue mirror provided by Shaoxing Cat's Claw Garden was used as the source of germplasm.

[0027] Carry out germplasm in vitro preservation test according to technical route of the present invention, detailed steps are as follows:

[0028] A method for in vitro preservation of germplasm resources of the succulent plant Blue Mirror of the genus Tilapia, characterized in that it comprises the following steps,

[0029] (1) Preparation of culture medium: Put the special tank for in vitro preservation of blue mirror germplasm resources into a sterilizing pot for high temperature and high pressure sterilization. Prepare the induction medium, the medium composition is MS+BA 1.0mg / L+TDZ 0.3mg / L. Prepare the differentiation medium, the medium composition is MS+BA 1.0mg / L+TDZ 0.5mg / L. Prepare the suppression medium, the composition of the suppression medium is 1 / 4MS+BA0.2 mg / L+NAA0.02 mg / L+2.5g / L mannitol. A proliferation medium was prepared, and the composition of the prol...

Embodiment 2

[0034] Example 2 is the same as Example 1 except for the inhibition of MS concentration in the culture medium. Example 2 The concentration of MS in the inhibition medium is 1 / 2 MS.

Embodiment 3

[0042] Example 3 is the same as Example 1 except for the inhibitor concentration in the inhibition medium. Example 3 The inhibitor concentration in the inhibition medium is 1.0 g / L mannitol.

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Abstract

The present invention belongs to the technical field of plant tissue culture fast propagation and germplasm resource preservation, and relates to a blue-green haworthia succulent plant (laworthia succulent plant lanjing) germplasm resource in-vitro preservation method. The blue-green haworthia succulent plant germplasm resource in-vitro preservation method comprises the following steps: preparingculture medium; preparing blue-green haworthia succulent plant germplasm resources; conducting in-vitro preservation; and conducting proliferation culture. The provided convenient and simple blue-green haworthia succulent plant germplasm resource in-vitro preservation method is low in preservation cost and simple in operation, and at the same time, concentration and storage conditions of the growth-inhibiting culture medium during the blue-green haworthia succulent plant germplasm resource in-vitro preservation are provided.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture rapid propagation and germplasm resource preservation, and relates to a method for in vitro preservation of the germplasm resources of the succulent plant of the genus Tilapia succulents. Background technique [0002] Blue mirror is a soft-leaved succulent plant in the genus Varida of the Liliaceae family, native to Africa. Its plant type is compact, short and strong, with a triangular window dome, top hairs, side hairs on both sides of the leaves, and two back hairs on the back of the leaves. The skin color is shiny, and it will produce different luster under different sunlight conditions. Because of its high ornamental value, it is quite popular in the market. However, due to the self-incompatibility of the plants of the genus, most of them are propagated by ramets and leaf cuttings, but these propagation methods are easy to damage the female parent, and the reproduction coefficien...

Claims

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Application Information

Patent Timeline
10 Apr 2020
Publication
CN110972936A
IPC
A01H4/00
CPC
A01H4/001; A01H4/005; A01H4/008
Inventors
邢海; 孙叶芳