Ultralow-temperature cryopreservation method for Yellow River carp sperms

A technology of ultra-low temperature freezing and preservation method, which is applied in the field of ultra-low temperature cryopreservation of Yellow River carp sperm, which can solve problems such as water pollution, damage to the ecological environment of the Yellow River, and population decline

Inactive Publication Date: 2020-04-10
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the impact of human activities and changes in the natural environment, such as water pollution, Yellow River dry-up and illegal fishing, the ecological environment of the Yellow River has been severely damaged, and the number of its population has declined rapidly. It is urgent to carry out the protection of germplasm resources

Method used

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  • Ultralow-temperature cryopreservation method for Yellow River carp sperms
  • Ultralow-temperature cryopreservation method for Yellow River carp sperms
  • Ultralow-temperature cryopreservation method for Yellow River carp sperms

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Embodiment

[0011] In order to achieve the purpose of ultra-low temperature cryopreservation of Yellow River carp sperm, this experiment is based on the above technical principles, through the dilution (D-14, D-15, D-16, D-17, D-19, D-20, D -21, Nonne's solution), diluted antifreeze final volume concentration (15%, 16%, 17%, 18%, 19%), diluted volume ratio (1:3, 1:4, 1:5, 1 :6, 1:7) and different equilibrium time and equilibrium distance, in order to obtain the best formula and procedure for preserving the sperm of Yellow River carp.

[0012] 1.1 Experimental reagents and materials

[0013] 1.1.1 Experimental reagents

[0014] Luteinizing hormone releasing hormone A2 for injection (Hangzhou Animal Pharmaceutical Factory), chorionic gonadotropin (Hangzhou Riyue Animal Pharmaceutical Co., Ltd.), sodium chloride (Sinopharm Chemical Reagent Co., Ltd.), potassium chloride (Tianli Tianli Chemical Reagent Co., Ltd.), Methanol (Tianjin Guangfu Science and Technology Development Co., Ltd.), Fish...

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Abstract

The invention discloses an ultralow-temperature cryopreservation method for Yellow River carp sperms. The method specifically comprises the following steps: preparing a sperm preserving fluid from anantifreeze agent methanol and a diluent; mixing the Yellow River carp seminal fluid and the sperm preserving fluid according to a volume ratio of 1: 5 in a cryopreservation tube, wherein the volume concentration of the antifreeze agent methanol in the mixed solution of the Yellow River carp semen and the sperm preservation solution is 17%; and then pre-cooling for 5 minutes at the temperature of 4DEG C, balancing for 12 minutes at the position 5 cm above the liquid nitrogen, putting into the liquid nitrogen and carrying out ultralow-temperature treatment for 1 day, whrein the diluent is prepared from the following raw materials in percentage by weight: 0.95 of NaCl, 0.05% of KCl, 1.5% of glucose and the balance of water. The ultralow-temperature cryopreservation technology for the YellowRiver carps is established, a technical guarantee is provided for establishment of a Yellow River carp sperm freezer and the technical support is provided for germplasm preservation and genetic breeding work of the Yellow River carps.

Description

technical field [0001] The invention belongs to the technical field of ultra-low temperature cryopreservation methods for sperm, and in particular relates to an ultra-low temperature cryopreservation method for Yellow River carp sperm. Background technique [0002] Yellow River carp (Cyprinus carpiohaematopterus) is a valuable fish resource in the Yellow River system in my country, an important freshwater aquaculture fish in Henan Province, and one of the four famous freshwater fish in my country. However, due to the impact of human activities and changes in the natural environment, such as water pollution, Yellow River dry-up and illegal fishing, the ecological environment of the Yellow River has been severely damaged, and its population has declined rapidly. It is urgent to carry out the protection of germplasm resources. [0003] In aquatic animals, the establishment of frozen gene bank is one of the important methods to protect germplasm resources. At present, scientist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A61D7/00A61D19/02
CPCA01N1/0221A01N1/0226A01N1/0284A61D7/00A61D19/021
Inventor 王磊董传举吴利敏马晓刘慧芬李学军李霞陈军平
Owner HENAN NORMAL UNIV
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