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Method for extending telomere of cell

A telomere extension and cell technology, applied in animal cells, cell culture medium, tumor/cancer cells, etc., can solve the problems of cumbersome cost and existing problems, and achieve the effect of excellent cost, low cost, time and efficiency

Active Publication Date: 2020-04-10
金贤锡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Korean Laid-Open Patent No. 10-2018-0123512 discloses a peptide derived from telomerase, which not only requires a synthesis process, but also is injected 3 times a week for two months, and is produced when measured one month later effect, so cumbersome and can be problematic in terms of cost

Method used

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  • Method for extending telomere of cell
  • Method for extending telomere of cell
  • Method for extending telomere of cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Induction of Telomere Elongation in Cells by Direct Ultrasonic Stimulation

[0067] Add 1 x 10 6 Directly apply 1W / cm to each cell 2 Cells stimulated with ultrasonic waves (20 KHz) for 5 seconds were cultured in a culture dish with the same medium for more than 1 day.

Embodiment 2

[0068] Example 2. Induction of Telomere Elongation in Cells by Direct Thermal Stimulation

[0069] 1 x 10 in 1 ml of hES medium 6 Each cell is put into a 1.5ml test tube (tube), exposed at a temperature of 50°C for 5 seconds, and then exposed at a temperature of 0°C for 10 seconds, and cultured with the same medium in a petri dish for more than 1 day.

Embodiment 3

[0070] Example 3. Induction of Telomere Elongation in Cells by Direct Light-Emitting Diode Light Stimulation

[0071] Add 1 x 10 to 1 ml of hES medium 6 The cells were directly stimulated by 808nm light-emitting diode laser (1W, 500Hz / sec) for 5 seconds and cultured with the same medium in a culture dish for more than 1 day.

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Abstract

Disclosed are a method for extending the telomeres of cells and cells having telomeres extended by physical stimuli, the method comprising the steps of: providing direct or indirect physical stimuli for cells; and incubating a mixture of the cells and a medium for a predetermined period of time, wherein the providing of direct physical stimuli refers to applying physical stimuli to a medium containing cells and the providing of indirect physical stimuli refers to applying physical stimuli to a cell-free medium which is then mixed with the cells. The method for extending telomeres of cells according to the present invention is simple and superior in terms of time, cost, effect efficiency, and safety, compared to conventional methods. Moreover, the method of the present invention induces cell division and has an anti-senescence effect in addition to extending telomeres and as such, can be expected to alleviate and prevent various senescence-associated diseases and symptoms as well as solving problems with shortened telomeres.

Description

technical field [0001] The present invention relates to a method for extending telomeres of cells, and more specifically, relates to a method for extending telomeres of the cells by directly or indirectly providing physical stimulation to the cells, and cells with telomeres extended by the above methods. Background technique [0002] Telomeres are DNA repeats at the ends of chromosomes (TTAGGG in humans). The telomere and the telomere protein complex (shelterin) combine to form a protective cover that regulates the expansion ability of cells and prevents the bonding between chromosomes and the loss of genetic information during cell division. DNA polymerase cannot amplify all 3' ends, so telomeres shorten by 30bp to 200bp each time a cell divides. If the telomere is shorter than the threshold and is close to the coding DNA and cannot maintain the telomere loop structure, the exposed telomere will be recognized by the p53 or p16INK4a signaling system, causing cell division t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00
CPCC12N13/00C12N2529/10C12N5/0018C12N2521/10C12N5/0625C12N5/0653C12N5/0656C12N5/0667C12N5/0693C12N5/0696C12N2523/00
Inventor 李镕丞
Owner 金贤锡
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