Holographic Micro Imaging Device

Abstract

A device for realizing holographic microscopic imaging, the device includes an inverted microscope and a holographic imaging device, wherein: the inverted microscope has an optical path entrance and an optical path exit, and the object to be observed is illuminated by a linearly polarized laser bright field, carrying the information of the object to be observed The light enters the inverted microscope through the optical path entrance, and the magnified holographic image of the object is obtained in the inverted microscope. The optical path exit of the inverted microscope is connected with the optical path entrance of the holographic imaging device; The transverse modes and polarization of the field are coupled and compounded for measurement. The quantitative phase imaging implementation device proposed by the present invention uses an imaging method of non-labeled specimens, which does not cause photobleaching of molecular structures due to light absorption caused by fluorescence imaging, overcomes the toxicity of excitation light to biological cells, and makes the present invention applicable to Watch the evolution of living cells.

Description

technical field [0001] The invention relates to the technical fields of microscopic imaging technology, interference imaging and phase imaging, in particular to a holographic microscopic imaging device. Background technique [0002] When the light field passes through the inhomogeneous medium, it will interact with the medium and modulate the wavefront and amplitude of the light. This process is called light scattering. Generally, only elastic scattering is considered, that is, the wavelength of light does not change after scattering. Since the amplitude and phase of the scattered light carry information about the internal structure of the scattering object, the structure of the object can be recovered by measuring the scattered field. This process is called the solution of the inverse scattering problem. This is the basis for tomographic imaging of transparent biological tissue or cells. Since intracellular tissue has many highly dynamic structures that are difficult to s...

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