Application of β-glucan and succinoglycan in the control of soil-borne fungal diseases
A technology of succinoglycans and soil-borne fungi, applied in the fields of application, chemicals for biological control, fungicides, etc., can solve the problems of different action mechanisms, achieve high feasibility, good selective enrichment, The effect of low economic cost
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Embodiment 1
[0030] Determination results of relative abundance of Streptomyces in typical soil
[0031] The original forest land in Enshi Prefecture, Hubei Province, the rotating cultivated land in Nanjing City, Jiangsu Province, the potato continuous cropping land in Tengzhou City, Shandong Province, and the tobacco continuous cropping land in Kunming City, Yunnan Province were selected as test areas. The mixture powder of beta-glucan and succinylglycan (laminarin:succinylglycan=1:1 w / w) was added to the selected soil surface layer, and the usage amounts were 0%, 0.05%, and 0.25%, respectively. Then, sampling is performed every 15d, and it ends at 60d. The sampled soil was extracted with Ezup column soil DNA extraction kit (Songgong Bio (Shanghai)) to extract total soil DNA and performed soil metagenomic sequencing. According to the sequencing results, the relative abundance of Streptomyces sequences in soil microflora was analyzed. figure 1 The relative abundances of Streptomyces in t...
Embodiment 2
[0033] Changes of β-glucanase, cellulase and chitinase activities in soil
[0034] Rotational cultivated land in Nanjing City, Jiangsu Province was selected as the test soil sample, and the mixture powder of β-glucan and succinylglycan (yeast glucan:succinylglycan = 1:10w / w) was added to the selected soil surface layer, using The amounts are 0%, 0.05%, and 0.25%, respectively. Then, sampling is performed every 15d, and it ends at 60d. Using laminarin, sodium carboxymethyl cellulose and chitin colloid as substrates, the hydrolase activity of soil leachate was measured at pH 5.5, the reaction temperature was 35℃, and the reaction time was 24h. Figure 2-Figure 4 It is the trend of the three hydrolase activities in soil with time. It can be seen from the figure that after adding the mixture of β-glucan and succinylglycan, the activities of the three enzymes all increased significantly with time and the addition amount of the mixture. The results showed that adding a mixture of...
Embodiment 3
[0036] Soil fungal content determination results
[0037] The potato continuous cropping field in Tengzhou City, Shandong Province was selected as the soil sample, and the mixture of β-glucan and succinyl polysaccharide (lentinan:succinyl polysaccharide=1:0.2w / w) was prepared into a solution with a concentration of 25g / L, Then sprayed to the selected soil surface, the application amount was 0%, 0.05%, 0.25%. Then, sampling is performed every 15d, and it ends at 60d. Then, the change process of the abundance of culturable fungi in the soil was determined by the dilution plate counting method: take 1 g of soil sample and dilute it into 10 mL of sterile phosphate buffer, shake vigorously for 30 minutes to make the soil evenly dispersed in the buffer, and then carry out gradient dilution , and finally choose the appropriate concentration to spread to PDA plate, and count after 48h incubation at 30℃. Figure 5 As shown, the fungal content in the soil decreased gradually with the ...
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