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Application of beta-glucan and succinyl glycan in prevention and control of soil-borne fungal diseases

A technology of succinoglycans and soil-borne fungi, applied in the fields of application, chemicals for biological control, fungicides, etc., can solve problems such as different mechanisms of action, and achieve high feasibility, good selective enrichment, and The effect of low economic cost

Active Publication Date: 2020-04-17
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to their different mechanisms of action

Method used

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  • Application of beta-glucan and succinyl glycan in prevention and control of soil-borne fungal diseases
  • Application of beta-glucan and succinyl glycan in prevention and control of soil-borne fungal diseases
  • Application of beta-glucan and succinyl glycan in prevention and control of soil-borne fungal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Determination results of relative abundance of Streptomyces in typical soil

[0034] The primeval forest land in Enshi Prefecture, Hubei Province, the rotational cropland in Nanjing City, Jiangsu Province, the continuous potato cropping land in Tengzhou City, Shandong Province, and the continuous tobacco cropping land in Kunming City, Yunnan Province were selected as test areas. The mixture powder of β-glucan and succinoglycan (laminaran:succinoglycan=1:1w / w) was added to the selected soil surface, and the usage amounts were 0%, 0.05%, and 0.25%, respectively. Then, take a sample every 15d and end at 60d. The sampled soil was extracted with the Ezup Column Soil DNA Extraction Kit (Sangon Biotech (Shanghai)) for total soil DNA and soil metagenomic sequencing. According to the sequencing results, the relative abundance of Streptomyces sequences in the soil microbial flora was analyzed. Figure 1-Figure 4 The trends of the relative abundance of Streptomyces in the above ...

Embodiment 2

[0036] Changes of activities of β-glucanase, cellulase and chitinase in soil

[0037] Select the rotation cultivated land in Nanjing City, Jiangsu Province as the test soil sample, add β-glucan and succinoglycan (yeast glucan: succinoglycan = 1:10w / w) mixture powder to the selected soil surface, use The amounts are 0%, 0.05%, 0.25%, respectively. Then, take a sample every 15d and end at 60d. Using laminarin, sodium carboxymethyl cellulose and chitin colloid as substrates, the hydrolase activity of the soil extract was measured at pH 5.5, the reaction temperature was 35°C, and the reaction time was 24 hours. Figure 5-Figure 7 It is the change trend of the three hydrolytic enzyme activities in the soil with time. It can be seen from the figure that after the mixture of β-glucan and succinoglycan was added, the activities of the three enzymes all increased significantly with time and the amount of the mixture added. The results showed that adding the mixture of β-glucan and s...

Embodiment 3

[0039] Soil fungus content determination results

[0040] A potato continuous cropping field in Tengzhou City, Shandong Province was selected as a soil sample, and a mixture of β-glucan and succinoglycan (lentinan: succinoglycan=1:0.2w / w) was prepared into a solution with a concentration of 25g / L. Then spray to the selected soil surface, and the dosage is 0%, 0.05%, 0.25%. Then, take a sample every 15d and end at 60d. Then, the abundance change process of cultivable fungi in the soil was determined by the dilution plate counting method: take 1 g of soil sample and dilute it into 10 mL of sterile phosphate buffer, shake vigorously for 30 minutes to make the soil evenly dispersed in the buffer, and then carry out gradient dilution , and finally select the appropriate concentration and spread it on the PDA plate, and count after culturing at 30°C for 48 hours. Figure 8 As shown, the content of fungi in the soil gradually decreased with the increase of the addition time and pol...

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Abstract

The invention discloses an application of beta-glucan and succinyl glycan in prevention and control of soil-borne fungal diseases. A method comprises the following steps: adding a mixture of beta-glucan and succinyl glycan into soil; so that the increase of the quantity of streptomyces in soil and the increase of the activity of hydrolase such as beta-glucanase, chitinase and cellulase in the soilare induced, and effectively prevent and control on various soil-borne pathogenic fungus diseases through the combined action of streptomyces and various hydrolases can be realized. The method has the advantages of wide sources of required raw materials, simple operation, better effect than single use of one polysaccharide, and good environmental compatibility.

Description

technical field [0001] The invention belongs to the technical field of biological control, and relates to the application of β-glucan and succinoglycan in the prevention and control of soil-borne fungal diseases. Background technique [0002] Soil-borne diseases refer to a type of disease in which pathogens originate from the soil and attack plants from the roots or stems of plants. They have the characteristics of wide distribution, severe damage, wide host range, multiple transmission routes, and long survival time. They have extremely harmful effects on plants. Great danger. Prevention and control of soil-borne diseases through biological methods is a kind of technology with low environmental risk. Among them, biological control by adding environmentally harmless inducers from exogenous sources mainly relies on the competitive growth, antagonism, and parasitism of the soil microbial population itself, which has minimal damage to the ecological environment and has high fe...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00
CPCA01N43/16
Inventor 李晶朱庭锐张建法
Owner NANJING UNIV OF SCI & TECH
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