Immune cell in-vitro induction amplification method

A technology of immune cells and nuclear cells, which is applied in the field of cell culture, can solve the problems of low expansion multiple, poor immune effect, complicated culture system, etc., achieve high expansion multiple, good effect, and reduce safety risks

Pending Publication Date: 2020-04-17
杭州恩格生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the technical problems existing in the prior art that the culture system for in vitro induction and expansion of immune cells is complicated, the amplification factor is not high, the immune effect is not good, and there are safety risks, the present invention provides an in vitro induction and expansion method for immune cells

Method used

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  • Immune cell in-vitro induction amplification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment A1～3

[0028] Embodiment A1~3: the configuration of immune cell activation medium

Embodiment A1

[0030] Use OpTmizer TMCTSTM serum-free medium as the basal medium, add relevant components to it, and make the components contained in the basal medium and their final concentrations: 8 volume percent autologous plasma, 500U / mL γ-interferon, 800U / mL interleukin-2 and 0.008KE / mL sapelin.

Embodiment A2

[0032] Use SuperCulture TmL500 human lymphocyte serum-free medium as the basal medium, add relevant components to it, and make the components contained in the basal medium and their final concentrations: 10 volume percent autologous plasma, 600U / mL γ-interference element, 1000U / mL interleukin-2 and 0.01KE / mL sapelin.

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PUM

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Abstract

The invention discloses an immune cell in-vitro induction amplification method in the related technical field of cell culture. The method comprises the following steps: producing a coated culture container; putting mononuclear cells of immune cells obtained by separation into the coated culture container, and carrying out immune cell activation culture for 24-36 h to obtain activated immune cells;preparing immune cells for induction amplification; differentiating and culturing to prepare differentiated immune cells; and preparing immune cells subjected to large-scale amplification, and performing passage once every 2-3 d under the condition of controlling the cell density to be 0.8-1.2 * 10<6 >/mL. The immune cell in-vitro induction amplification culture system is clear, and the amplification multiple is high; and the immune cells prepared by the immune cell in-vitro induction amplification method are good in immune effect, and the safety risk is effectively reduced.

Description

technical field [0001] The invention relates to the technical field related to cell culture, in particular to a method for inducing and expanding immune cells in vitro. Background technique [0002] Biological immune cell therapy is through in vitro culture, proliferation, activation, and infusion back into the body to induce an autologous antiviral immune response, so that after the human antiviral immunity is activated, antiviral substances are continuously produced to kill the virus. Immune cells have a strong ability to recognize tumor cells and bacteria, like "cell missiles", which can accurately "shoot" tumor cells or bacteria without harming "innocent" normal cells. It is a very effective treatment method . [0003] However, in the prior art, the culture system for the in vitro induction and expansion of immune cells is complicated, the amplification factor is not high, and the immune effect is not good, so there are safety risks. Contents of the invention [0004...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783C12N5/0784A01N1/02
CPCC12N5/0639C12N5/0646A01N1/0221C12N2501/599C12N2533/50C12N2501/24C12N2501/2302C12N2501/2301C12N2500/90
Inventor 王健侯雷何灿洋何灿平竺亚斌
Owner 杭州恩格生物医疗科技有限公司
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