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Method for removing intracellular active oxygen species

A technology of active oxygen and cells, applied in biochemical equipment and methods, using electricity/wave energy to treat microorganisms, electricity/wave energy to treat enzymes, etc., can solve problems such as toxic and side effects

Active Publication Date: 2020-04-17
河南省医药科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned methods all need to swallow drugs or nanoparticles, but the taking of drugs has certain toxic and side effects.

Method used

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  • Method for removing intracellular active oxygen species
  • Method for removing intracellular active oxygen species
  • Method for removing intracellular active oxygen species

Examples

Experimental program
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Embodiment 1

[0019] 1. Prepare cell samples for confocal observation (take umbilical vein endothelial cells HUVEC as an example) HUVEC cells 1x10 5 Each species was placed in a laser confocal small dish (35 mm in diameter) and cultured in a cell culture box for 24 hours. The supernatant was discarded, washed twice with PBS, and the cells were incubated with a reactive oxygen species (ROS) kit at a reagent concentration of 0.8 μm / L for 25 minutes. After washing with PBS three times and adding Hank's balanced salt solution (10 mL) to incubate at room temperature for 40 minutes, the ROS content in the cells reached a stable equilibrium state.

[0020] 2. Ultrasonic radiation cells at different times

[0021] in such as figure 1 In the device shown, the input power of the ultrasonic signal generator is 0.2W, the ultrasonic radiation time is set to 1s, 3s, 5s, the ultrasonic frequency is 1MHZ, and the diameter of the ultrasonic transducer is 15mm.

[0022] During the irradiation process, the...

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Abstract

The invention provides a method for removing intracellular active oxygen species, and relates to the technical field of active oxygen species removal. According to the method disclosed by the invention, the ROS in the cells is cleaned by only adopting the action of high-frequency ultrasonic radiation, and other auxiliary reagents or particles do not need to be added; and during ultrasonic radiation, the ultrasonic frequency is 1MHZ, and the power and the time are adjustable. In the embodiment of the invention, under the action of ultrasonic radiation, the intracellular ROS content is reduced,namely, intracellular ROS is cleaned; and along with the prolonging of the ultrasonic radiation time or the increase of the power, the ROS removal amount is increased, which indicates that the ultrasonic radiation can realize the cleaning of the ROS in the cells.

Description

technical field [0001] The invention belongs to the technical field of removing active oxygen, and in particular relates to a method for removing active oxygen in cells. Background technique [0002] Free radicals in the human body are divided into oxygen free radicals and non-oxygen free radicals. Oxygen free radicals include hydrogen peroxide molecules, hydroxyl free radicals, peroxyl free radicals, alkoxyl free radicals, superoxide anion free radicals, etc. They are collectively called reactive oxygen species (ROS), which are the most important species in the human body. of free radicals. The generation and removal of reactive oxygen species in organisms are in a state of dynamic balance. When various factors break this balance and cause the concentration of reactive oxygen species to exceed the physiological limit, biological macromolecules will be damaged, including lipid peroxidation, DNA oxidative damage, and protein oxidation. And monosaccharide oxidation, etc., re...

Claims

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Application Information

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IPC IPC(8): C12N13/00
CPCC12N13/00
Inventor 张丽果刘晓洁汲振余代丽萍赵阳何美霞彭颖张壮丽刘静静杨洋完迪迪高璐张灿吉云杨小静裘一兵
Owner 河南省医药科学研究院