Method for cultivating bacterial root seedlings of tung oil trees
A cultivation method and technology for mycorrhizal seedlings, which are applied in the field of cultivating tung tree mycorrhizal seedlings, can solve problems such as limited role in promoting seedling cultivation, and achieve the effects of convenient transportation, improved high-diameter growth, and reduced transportation costs.
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Embodiment 1
[0025] A method for cultivating tung mycorrhizal seedlings, comprising the steps of:
[0026] S1, preparation of culture substrate
[0027] Peat, vermiculite and perlite were selected as the raw materials of the culture substrate and sterilized. The volume ratio of peat, vermiculite and perlite was 3:2:1; the culture substrate was sterilized twice at 121°C each time. The sterilization time is 2 hours; the interval between two sterilizations is 1-2 days; the sterilized culture medium is put into a seedling cultivation container with a volume of 250 milliliters.
[0028] S2, Tung seed treatment
[0029] The tung oil tung seeds are first treated with sand storage to accelerate germination. When 2 to 3 true leaves grow and the height of the seedlings is 5cm, they are transplanted into a seedling container equipped with a culture medium. For the integrity of the root system, the AM fungal inoculant is inserted at the same time as transplanting;
[0030] Wherein, the AM fungal ag...
Embodiment 2
[0035] In order to verify the effect of a kind of tung tree mycorrhizal seedling cultivation method of the present invention, inoculate the indigenous AM fungus (4a) proposed by the application, inoculate the AM fungal agent (endo) introduced in the U.S. and non-inoculated arbuscules respectively in the cultivation process Mycorrhizal fungi were detected on tung tree seedlings:
[0036] As shown in Table 1,
[0037] After the seedlings were inoculated with AM fungi test for 5 months, the seedling growth of each treatment was investigated. As a result, the seedling height and ground diameter of the inoculated native AM fungi (4a) were 60.53cm and 9.32mm respectively, and the AM fungal agent ( endo) were 35.47cm and 9.46mm in height and ground diameter respectively, while the control without inoculation had only 45.47cm and 8.79mm in height and ground diameter.
[0038] It can be seen that the inoculation of indigenous AM fungi significantly improved the height growth of tung t...
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