Applications of genistein-copper coordination compound in preparation of drugs for treating breast cancer
A technology of copper complexes and genistein, applied in the field of medicine, can solve the problem of low anti-tumor activity and achieve the effect of small growth inhibition, high safety, and significant selective inhibition
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0028] 1. Preparation of genistein-copper complexes
[0029] Weigh 5.40g of genistein (0.02mol), add 170mL of absolute ethanol, heat until the genistein is completely dissolved, adjust the pH to 8 with ammonia water, and add 4.12g of CuCl after the pH is stable 2 2H 2 O (0.024mol), refluxed in a constant temperature water bath at 60°C for 7h; after the reaction, the reaction solution was cooled to room temperature, centrifuged at 3000 rpm for 2 minutes, the precipitate was washed three times with 50% ethanol solution, and then vacuum-dried at 50°C to obtain 4.878 g grass green powder, the yield is 81%.
[0030] 2. Preparation of genistein-zinc complex
[0031] Weigh 5.40g of genistein (0.02mol), add 170mL of absolute ethanol, heat until the genistein is completely dissolved, adjust the pH to 8 with ammonia water, add 4.39g of zinc acetate (0.024mol) after the pH value is stable, and keep the temperature at 60°C Heat and reflux in a water bath for 7 hours; after the reaction...
Embodiment 1
[0047] Example 1 Effect of Genistein-Copper Complex on the Growth of Human Breast Cancer Cell MCF-7
[0048]Human breast cancer cell MCF-7 was treated with DMEM+10% fetal bovine serum+1% penicillin and streptomycin in 5% CO 2 , Cultivated in a saturated humidity incubator at 37°C, subcultured once every 2-3 days, cultured two generations of cells after recovery, took the cells in the logarithmic growth phase, and prepared them with a medium containing 10% FBS at a concentration of 5×10 4 / mL of cell suspension, inoculate into 96-well culture plate, add 100 μL to each well, in 5% CO 2 , Continue culturing for 24 hours in a saturated humidity incubator at 37°C;
[0049] Genistein, genistein-copper complex, genistein-zinc complex, and 5-fluorouracil (positive control) were prepared in DMSO to a final concentration of 16, 32, 64, and 128 μM, respectively, and 200 μL was added to each well. An equal volume of DMSO was used as a blank control, and three auxiliary wells were set up...
Embodiment 2
[0056] Example 2 Effect of Genistein-Copper Complex on the Growth of Human Umbilical Vein Endothelial Cells HUVEC
[0057] Human umbilical vein endothelial cells HUVEC were treated with DMEM + 10% fetal bovine serum + 1% penicillin and streptomycin in 5% CO 2 , Cultivated in a saturated humidity incubator at 37°C, subcultured once every 2 to 3 days, cultured two generations of cells after recovery, took the cells in the logarithmic growth phase, and prepared them with a medium containing 10% FBS at a concentration of 5×10 4 / mL of cell suspension, inoculate into 96-well culture plate, add 100 μL to each well, in 5% CO 2 , Continue culturing for 24 hours in a saturated humidity incubator at 37°C;
[0058] Genistein, genistein-copper complex, genistein-zinc complex, and 5-fluorouracil (positive control) were prepared with DMSO to a final concentration of 16, 32, 64, and 128 μM, respectively, and 200 μL was added to each well. An equal volume of DMSO was used as a blank control...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More