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Method for rapid inoculation of seedling leaves with barley yellow mosaic virus

A barley and yellow flower technology, applied in the agricultural field, can solve the problems of large experimental errors, fungal vector infection efficiency, and complexity, and achieve the effects of less damage to the inoculated leaves, maintaining virus activity, and less impact

Active Publication Date: 2020-04-28
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the construction of the system is very complicated, and the fungal mediator also has the problem of infection efficiency, and the experimental error is large

Method used

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  • Method for rapid inoculation of seedling leaves with barley yellow mosaic virus
  • Method for rapid inoculation of seedling leaves with barley yellow mosaic virus
  • Method for rapid inoculation of seedling leaves with barley yellow mosaic virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of indoor rapid inoculation method of barley yellow mosaic seedling stage, comprises the following steps:

[0030] (1) Collection and preservation of diseased leaves

[0031] During the peak period of barley yellow mosaic disease, a single plant with typical disease characteristics was selected from the susceptible materials in the disease nursery, and the green leaves with obvious barley yellow mosaic disease spots were taken and stored in a -80°C ultra-low temperature refrigerator for later use.

[0032] (2) Preparation of reagents and utensils

[0033] Configure K 2 HPO 4 Buffer solution (0.1mol / L, pH=9.1), placed at 4°C for later use; mix 150-mesh and 200-mesh quartz sand, cotton gloves, mortar and pestle at a volume ratio of 1:1, and sterilize at high temperature for later use use.

[0034] (3) Virus preparation

[0035] Place the mortar on ice to pre-cool, add 5-6 thawed diseased leaves (about 1.0g), 1.0ml K 2 HPO 4 Buffer solution and 0.5g of quartz...

Embodiment 2

[0044] In 2019, the present invention relates to a barley yellow mosaic virus indoor friction inoculation method at the seedling stage of barley, and compares it with the conventional juice friction inoculation method:

[0045] (1) Collection and preservation of diseased leaves

[0046] On March 5, 2019, at the barley yellow mosaic disease resistance identification disease nursery of Yangzhou University, the leaves of the "Dan Er" plant with typical barley yellow mosaic symptoms were collected, collected and placed in a -80°C ultra-low temperature refrigerator.

[0047] (2) Conventional juice friction inoculation

[0048] On August 21 and September 2, 2019, conventional juice friction inoculation was used to inoculate the "Dan Er" seedlings in the two-leaf and one-heart stage. The inoculation was repeated one week later, and RNA was extracted one month after the first inoculation for virus identification.

[0049] (3) Inoculation method of the present invention

[0050] On Se...

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Abstract

The invention belongs to the technical field of agriculture, relates to a plant disease inoculation technique, and particularly relates to a method for rapid inoculation of seedling leaves with barleyyellow mosaic virus. The method comprises the following steps: selecting barley with characteristics of barley yellow mosaic virus disease, selecting green leaves with obvious scabs caused by the barley yellow mosaic virus disease, and storing the green leaves at low temperature for further use; mixing the thawed diseased leaves with K2(subscript)HPO4(subscript) buffer solution and quartz sand, and rolling the diseased leaves for juicing to obtain virus juice; and using a cotton glove for dipping the virus juice and the quartz sand, clamping the leaves from base parts of the leaves, and sliding fingers to tip parts of the leaves to finish inoculation. The method adopts the cotton glove to clamp the leaves for rapid rubbing inoculation, and can simplify the operation process, provide highsensitivity and improve inoculation efficiency on the premise of not influencing the normal growth of the inoculated seedlings.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to plant disease inoculation technology, in particular to a rapid inoculation method for barley yellow mosaic virus seedling leaves. Background technique [0002] Barley yellow mosaic disease is one of the main diseases in the Eurasian winter barley region. It often occurs in the middle and lower reaches of the Yangtze River and the Huanghuai winter barley region in my country, especially in Jiangsu, Zhejiang and Shanghai. Barley yellow mosaic virus is a viral disease caused by barley yellow mosaic virus (BaYMV), barley mild mosaic virus (BaMMV) or their complex. Under field conditions, the virus uses Polymyxa graminis in the soil as a transmission medium, and infects and becomes diseased from the roots of barley. Because the virus resides in fungal media and survives in the soil for a long time, it is difficult to control barley yellow mosaic disease by chemical methods. Excavat...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G22/20
CPCA01G7/06A01G22/20
Inventor 潘雨涵许如根吕超郭宝健王菲菲
Owner YANGZHOU UNIV