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A kind of rapid seedling leaf inoculation method of barley yellow mosaic virus

A barley and yellow flower technology, applied in the agricultural field, can solve the problems of fungal vector infection efficiency, large experimental error, complexity, etc., and achieves the effect of maintaining virus activity, inoculating foliar damage and small impact.

Active Publication Date: 2022-04-22
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the construction of the system is very complicated, and the fungal mediator also has the problem of infection efficiency, and the experimental error is large

Method used

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  • A kind of rapid seedling leaf inoculation method of barley yellow mosaic virus
  • A kind of rapid seedling leaf inoculation method of barley yellow mosaic virus
  • A kind of rapid seedling leaf inoculation method of barley yellow mosaic virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of indoor rapid inoculation method of barley yellow mosaic seedling stage, comprises the following steps:

[0030] (1) Collection and preservation of diseased leaves

[0031] During the peak period of barley yellow mosaic disease, a single plant with typical disease characteristics was selected from the susceptible materials in the disease nursery, and the green leaves with obvious barley yellow mosaic disease spots were taken and stored in a -80°C ultra-low temperature refrigerator for later use.

[0032] (2) Preparation of reagents and utensils

[0033] Configure K 2 HPO 4 Buffer solution (0.1mol / L, pH=9.1), placed at 4°C for later use; mix 150-mesh and 200-mesh quartz sand, cotton gloves, mortar and pestle at a volume ratio of 1:1, and sterilize at high temperature for later use use.

[0034] (3) Virus preparation

[0035] Place the mortar on ice to pre-cool, add 5-6 thawed diseased leaves (about 1.0g), 1.0ml K 2 HPO 4 Buffer solution and 0.5g of quartz...

Embodiment 2

[0044] In 2019, the present invention relates to a barley yellow mosaic virus indoor friction inoculation method at the seedling stage of barley, and compares it with the conventional juice friction inoculation method:

[0045] (1) Collection and preservation of diseased leaves

[0046] On March 5, 2019, at the barley yellow mosaic disease resistance identification disease nursery of Yangzhou University, the leaves of the "Dan Er" plant with typical barley yellow mosaic symptoms were collected, collected and placed in a -80°C ultra-low temperature refrigerator.

[0047] (2) Conventional juice friction inoculation

[0048] On August 21 and September 2, 2019, conventional juice friction inoculation was used to inoculate the "Dan Er" seedlings in the two-leaf and one-heart stage. The inoculation was repeated one week later, and RNA was extracted one month after the first inoculation for virus identification.

[0049] (3) Inoculation method of the present invention

[0050] On Se...

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Abstract

The invention belongs to the field of agricultural technology, and relates to plant disease inoculation technology, in particular to a method for quickly inoculating barley yellow mosaic virus seedling leaves, comprising the following steps: selecting barley with the characteristics of barley yellow mosaic disease, and taking the barley yellow mosaic virus The green leaves with obvious lesion spots should be stored at low temperature for later use; the thawed diseased leaves should be mixed with K 2 HPO 4 The buffer solution and quartz sand are mixed, and the diseased leaves are crushed to make the juice out to obtain virus juice; use cotton gloves to dip the virus juice and quartz sand, clamp the leaf from the base of the leaf to the tip of the leaf and slide to complete the inoculation. The method adopts cotton glove to fasten frictional inoculation, can simplify the operation process, has high sensitivity and improves inoculation efficiency under the premise of not affecting the normal growth of inoculated seedlings.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to plant disease inoculation technology, in particular to a rapid inoculation method for barley yellow mosaic virus seedling leaves. Background technique [0002] Barley yellow mosaic disease is one of the main diseases in the Eurasian winter barley region. It often occurs in the middle and lower reaches of the Yangtze River and the Huanghuai winter barley region in my country, especially in Jiangsu, Zhejiang and Shanghai. Barley yellow mosaic virus is a viral disease caused by barley yellow mosaic virus (BaYMV), barley mild mosaic virus (BaMMV) or their complex. Under field conditions, the virus uses Polymyxa graminis in the soil as a transmission medium, and infects and becomes diseased from the roots of barley. Because the virus resides in fungal media and survives in the soil for a long time, it is difficult to control barley yellow mosaic disease by chemical methods. Excavat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06A01G22/20
CPCA01G7/06A01G22/20
Inventor 潘雨涵许如根吕超郭宝健王菲菲
Owner YANGZHOU UNIV