Crocodile antioxidant peptide complex, and preparation method and applications thereof

A technology of antioxidant peptides and complexes, applied in the preparation method of peptides, chemical instruments and methods, applications, etc., can solve problems such as toxic side effects, use restrictions, etc.

Active Publication Date: 2020-05-01
福建鼍龙实业有限责任公司
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical antioxidants are the most produced, but t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Crocodile antioxidant peptide complex, and preparation method and applications thereof
  • Crocodile antioxidant peptide complex, and preparation method and applications thereof
  • Crocodile antioxidant peptide complex, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] The preparation of crocodile antioxidant peptide complex, the steps are as follows:

[0110] After washing with 100g of ultrapure water and drying with filter paper, add 15-20 times the volume of ultrapure water (1500mL-2000mL), and cook at high temperature for 25min-35min. Using papain, under its optimal enzymolysis conditions (55°C, enzyme addition 3.00% (w / w), substrate concentration 6.5% (w / v)) enzymolysis 4h, boiling water bath inactivation 15min, and then Filter with 4 layers of gauze to obtain the enzymatic hydrolyzate of crocodile antioxidant peptide complex. The above enzymolysis solution was centrifuged at 12,000 rpm for 30 minutes at room temperature, and the supernatant was collected and freeze-dried to obtain crocodile antioxidant peptide complex powder.

Embodiment 2

[0112] Separation and purification of the crocodile antioxidant peptide complex obtained in Example 1

[0113] Ultrafiltration: redissolve the crocodile antioxidant peptide complex powder in ultrapure water, and grade through ultrafiltration membranes with molecular weight cut-offs of 100kDa, 5kDa, and 2kDa in sequence, and determine the hydrolyzate of 100kDa hydrolyzate four components ABTS + · Free radical scavenging rate. The result is as Figure 5 As shown, it can be seen that the component crocodile antioxidant peptide-1 has the highest clearance rate, so the component is collected, freeze-dried, and subjected to the next step of anion exchange chromatography separation and purification.

[0114] Anion exchange chromatography: Capto TM Q-pillar (HiTrap TM , 5mL) fully equilibrated with 20mM Tris-HCl (pH9.0) buffer in advance; After mixing, use a 2mL syringe to load the sample, and the sample volume is 2.0mL; The buffer is eluted stepwise. Elution profile as Ima...

Embodiment 3

[0122] Research on the antioxidant activity of the crocodile antioxidant peptide complex obtained in Example 1.

[0123] 3.1. The ability to scavenge DPPH free radicals

[0124] The antioxidant capacity in vitro was studied by measuring the free radical scavenging rate of 1,1-diphenyl-2-trinitrophenylhydrazyl (1-diphenyl-2-picrylhydrazyl, DPPH). Take 0.1ml of different concentrations of crocodile antioxidant peptide complex (0mg / mL, 0.5mg / mL, 1.0mg / mL, 1.5mg / mL, 2.0mg / mL, 2.5mg / mL, 3.0mg / mL), add 0.1 ml 0.2mM DPPH·solution, mix well, stand in the dark for 30min, then measure the absorbance at 517nm ultraviolet wavelength. The results are shown in Table 2 below:

[0125] DPPH free radical scavenging rate calculation formula:

[0126]

[0127] Among them, A c Absorbance value of 0.1mL ultrapure water + 0.1mL DPPH solution; A i It is the absorbance value of 0.1mL sample + 0.1mL DPPH solution; A j It is the absorbance value of 0.1mL ultrapure water + 0.1mL absolute ethano...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a crocodile antioxidant peptide complex. The complex includes, by mass percentage, 0.1-2% of crocodile antioxidant peptide 1, 0.5-3% of crocodile antioxidant peptide 2, 0.1-2%of crocodile antioxidant peptide 3, 0.1-2% of crocodile antioxidant peptide 4 and 0.5-5% of crocodile antioxidant peptide 5; and the molecular weight of the complex is in the range of 800-1,500 Da. The complex can effectively eliminate DPPH.free radicals, ABTS<+>.free radicals and hydroxyl(OH<->)free radicals; and the complex has no toxic action on Lo2 and HaCat cells and has repairing functions on the HaCat cells injured by oxidation induced by hydrogen peroxide.

Description

technical field [0001] The present application relates to the field of biological products, in particular to a crocodile antioxidant peptide complex and its preparation method and application. Background technique [0002] Crocodile (Crocodile) is a vertebrate amphibian reptile with a life history of more than 200 million years. It is one of the oldest and most primitive animals discovered so far, and is known as a "living fossil". Crocodiles are full of treasures, with high medicinal value, food value and economic value. For example, crocodile meat is rich in protein, and can reduce phlegm and relieve cough, nourish the heart and lungs; crocodile bone can be used to prevent and treat rheumatic bone pain, senile osteoporosis and children's rickets; crocodile gall has antipyretic, calming heat, and promotes metabolism It also has a significant effect on the treatment of fatty liver and cirrhosis; eating crocodile liver can detoxify eyesight, nourish blood and benefit the liv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K7/06C07K7/08C07K1/36C07K1/34C07K1/18C07K1/16C07K1/20A61K8/64A61Q19/08
CPCC07K7/06C07K7/08A23L33/18A61K8/64A61Q19/08A23V2002/00A23V2200/318A23V2250/55
Inventor 张紫然叶沁陈清西李华亮熊佑熊
Owner 福建鼍龙实业有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap