Recombinant hansenula polymorpha cell disruption buffer solution for expressing hand-foot-and-mouth disease vaccine antigen as well as preparation method and application thereof

Abstract

The embodiment of the invention discloses a recombinant hansenula polymorpha cell disruption buffer solution for expressing a hand-foot-and-mouth disease vaccine antigen as well as a preparation method and application of the recombinant hansenula polymorpha cell disruption buffer solution. The cell disruption buffer solution is prepared from the following components: 1.00 g / L to 100.00 g / L of tromethamine, 10.00 g / L to 100.00 g / L of medicinal sodium chloride and 1.00 g / L to 100.00 g / L of glycerinum. By implementing the method disclosed by the invention, the breakage rate, the target protein content and the target antigen content of the recombinant hansenula polymorpha cell breakage product can be obviously improved.

Description

technical field [0001] The invention relates to the technical field of preparation of hand, foot and mouth disease vaccine expressed by recombinant Hansenula, in particular to a recombinant Hansenula cell breaking buffer expressing hand, foot and mouth disease vaccine antigen and its preparation method and application. Background technique [0002] During the preparation of the HFMD vaccine expressed by the recombinant Hansenula yeast, solid-liquid separation of the fermentation product and cell disruption are required, and the final goal is to collect the target protein and target antigen. The commonly used cell disruption method in the prior art is high-pressure homogenization, and a large amount of cell disruption buffer must be used in the process. The quality of the cell disruption buffer directly affects the cells in the preparation process of the hand, foot and mouth disease vaccine expressed by recombinant Hansenula. The crushing effect, target protein content, and t...

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Problems solved by technology

[0004] However, the inventor found in the process of implementing the present invention that under the same process conditions, high-pressure homogenization was used for crushing, and the tromethamine (Tris) buffer commonly used in the market was used for cell crushing, and the cell crushing product of recombinant Hansenula spp. Cell disruption rate, target protein content and target antigen content are not ideal

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