Method for extracting small molecule peptide from sea cucumber and application of small molecule peptide

A technology of small molecular peptides and sea cucumbers, applied in the biological field, can solve the problems of insufficient research on efficient extraction of small molecular peptides from sea cucumbers, low extraction efficiency, cumbersome steps, etc., to achieve the elimination of protein allergens, good storage stability, biological high potency effect

Pending Publication Date: 2020-05-12
吴伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, although people have done a lot of research on the extraction of small molecular peptides from sea cucumbers, most of the research is relatively simple, and the research on the efficient extraction of small molecular peptides from sea cucumbers is not deep enough.
Moreover, the existing methods for extracting small molecular peptides from sea cucumbers have problems such as low extraction efficiency, high cost or cumbersome steps.

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  • Method for extracting small molecule peptide from sea cucumber and application of small molecule peptide
  • Method for extracting small molecule peptide from sea cucumber and application of small molecule peptide

Examples

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Embodiment 1

[0034] This embodiment discloses a method for extracting small molecule peptides from sea cucumbers. The process flow chart is as follows figure 1 As shown, it specifically includes the following steps:

[0035] (1) Raw material pretreatment: Soak the fresh sea cucumbers in warm water at 40°C for 1 hour, and wash the salt and sediment impurities in the sea cucumbers several times until the cleaning solution has no salty taste.

[0036] (2) High temperature and high pressure extraction: use high temperature and high pressure cooking tank to extract, add pure water 3 times the weight of the raw material, and raise the temperature to 80-90℃, add the cleaned sea cucumber, soak while stirring, soak for 2h and then increase the pressure to 120℃ , 0.2MPa, constant temperature and pressure extraction for 3-4 hours, the extraction liquid is discharged into the hydrolysis tank, the sea cucumber residue is discharged, and the subsequent production is combined and extracted. This equipment is...

Embodiment 2

[0046] This embodiment discloses a method for extracting small molecule peptides from sea cucumbers, which specifically includes the following steps:

[0047] (1) Raw material pretreatment: Soak the fresh sea cucumbers in warm water at 40°C for 1 hour, and wash the salt and sediment impurities in the sea cucumbers several times until the cleaning solution has no salty taste.

[0048] (2) High temperature and high pressure extraction: use high temperature and high pressure cooking tank to extract, add pure water 4 times the weight of the raw material, heat up to 80-90°C, add the cleaned sea cucumber, soak while stirring, soak for 2 hours and then increase the pressure to 120°C , 0.2MPa, constant temperature and constant pressure extraction for 3-4 hours, the extraction liquid is discharged into the hydrolysis tank, the sea cucumber residue is discharged, and combined extraction with subsequent production. This equipment is used for extraction under stirring conditions, and the liqui...

Embodiment 3

[0058] This embodiment discloses a method for extracting small molecule peptides from sea cucumbers, which specifically includes the following steps:

[0059] (1) Raw material pretreatment: Soak the fresh sea cucumbers in warm water at 40°C for 1 hour, and wash the salt and sediment impurities in the sea cucumbers several times until the cleaning solution has no salty taste.

[0060] (2) High-temperature and high-pressure extraction: Use high-temperature and high-pressure cooking tanks to extract, add pure water 4 times the weight of the raw materials, heat up to 85°C, add the cleaned sea cucumbers, soak while stirring, soak for 2h and then increase the pressure to 120°C, 0.2 MPa, constant temperature and constant pressure extraction for 3.5 hours, the extraction liquid is discharged into the hydrolysis tank, the sea cucumber residue is discharged, and combined extraction with subsequent production. This equipment is used for extraction under stirring conditions, and the liquid-sol...

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Abstract

The invention belongs to the technical field of biologics and in particular relates to a method for extracting small molecule peptide from sea cucumber and application of the small molecule peptide. The method for extracting small molecule peptide from sea cucumber, which is provided by the invention, comprises the following steps: pretreating sea cucumber, performing extraction so as to obtain asea cucumber extract, performing step-by-step enzymolysis on the sea cucumber extract, performing decoloring and deodorization treatment, and performing purification treatment, so as to obtain a smallmolecule peptide solution. The small molecule peptide solution has the advantages of being good in water solubility, good in stability, good in emulsibility, low in viscosity, easy to digest and absorb, free of antigenicity, safe to eat, and the like.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a method for extracting small molecule peptides from sea cucumbers and its application. Background technique [0002] Sea cucumbers are marine echinoderms belonging to the Holothuroidea class of Echinodermata. There are about 1,100 species of sea cucumbers in the world, and about 40 species are edible. There are more than 140 kinds in our country, and about 20 kinds are edible. Its nutritional and medicinal value is extremely high. It is as famous as ginseng, bird's nest and shark's fin. It is one of the eight treasures in the world. According to "Supplements to Compendium of Materia Medica": sea cucumbers are sweet and salty, nourishes the kidneys, nourishes the essence, ingests urine, aphrodisiac and cures dysfunction, its nature warms up, enough for the enemy ginseng, hence the name sea cucumber. The organic composition of dried sea cucumber body wall contains protein (po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC07K1/145C07K1/34C12P21/06
Inventor 不公告发明人
Owner 吴伟
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