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Nematicidal active substance as well as preparation method and application thereof

An active substance and nematicide-killing technology, applied in botany equipment and methods, biochemical equipment and methods, nematicides, etc., can solve problems such as high toxicity, destroy ecological balance, threaten human health, etc., and achieve strong poisonous killing activity , the effect of strong stability

Active Publication Date: 2020-05-15
GUANGDONG ZHENGE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For a long time, the prevention and control of plant nematode diseases has mainly relied on chemical nematicides, but most of the nematicides are highly toxic and high-residual chemicals. While killing plant parasitic nematodes in the soil, they also kill beneficial organisms and seriously damage the ecological balance. pollute the environment and threaten human health

Method used

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  • Nematicidal active substance as well as preparation method and application thereof
  • Nematicidal active substance as well as preparation method and application thereof
  • Nematicidal active substance as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 3

[0057] Embodiment 3 NMR analysis of the structural formula of the nematicide active substance

[0058] Use a high-resolution nuclear magnetic resonance spectrometer to measure the nuclear magnetic resonance data of nematicidal active substances, detect the number of hydrogen atoms and carbon atoms and their correlations, obtain hydrogen and carbon spectra, and conduct spectral analysis to know the composition of the nucleus of this object Location and type, find the molecular structure of a substance, draw a structural image of a substance. In order to ensure the accuracy of the structure, the relative molecular mass was detected by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry.

[0059] Crystalline product, colorless crystals (methanol, CD 3 OD), 1H NMR (CD3OD, 400MHz) δ: 3.68 (each 3H, s, 7,8-CH3), 2.95, 2.85 (each 2H, AB system, d, J=12.0Hz, 2,4-CH2). 13C NMR(CD3OD,100MHz)δ:175.00(s,C-6),170.46(s,C-1,C-5),72.84(s,C-3),50.78(q,C-7,C- 8),...

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Abstract

The invention provides a nematicidal active substance, which is prepared from a secondary metabolite product of Aspergillus japonicus ZW1. The preparation method comprises the following steps: carrying out liquid fermentation culture on Aspergillus japonicus ZW1 capable of generating active substances for poisoning and killing nematodes to obtain a fermentation culture solution containing nematicidal active metabolites; obtaining a crude extract from the fermentation culture solution; and purifying the crude extract to obtain the nematicidal active substance. The provided nematicidal active substance has strong stability and strong poisoning activity on root-knot nematodes, the death rate of the root-knot nematodes at the concentration of 1.25 mg / mL for 48 hours is 90% or above, and the death rate of the root-knot nematodes at the concentration of 2mg / mL for 6 hours is 100%. Meanwhile, the death rate of soybean cyst nematodes is 100% at the concentration of 2mg / mL within 48h.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a nematicide active substance, in particular to a nematicidal active substance prepared from a secondary metabolite product of Aspergillus japonicus ZW1, and the preparation of the nematicide active substance Method and its use in plant biological control of pests. Background technique [0002] Root-knot nematode is a disease distributed worldwide, and it is one of the most important crop diseases in China at present, causing great economic harm. Since Berkeley first discovered root-knot on cucumbers in 1855, plant root-knot nematodes have only begun to receive people's attention, and its occurrence and damage have a history of more than 150 years. In recent years, with the rapid development of vegetable cultivation area, annual planting and long-distance transportation, a suitable environment has been provided for the occurrence, development and spread of root-knot nematode disease, ...

Claims

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Application Information

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IPC IPC(8): C07C69/704A01N37/36A01P5/00C12P7/62C12N1/14C12R1/66
CPCC07C69/704A01N37/36C12P7/62C12N1/14
Inventor 吴海燕王冬亚周勋波何琼
Owner GUANGDONG ZHENGE BIOLOGICAL TECH CO LTD