Cimaterol-resistant monoclonal antibody and application thereof
A monoclonal antibody, cimaterol technology, applied in the biological field, can solve the problems of impure antibody and slow detection of cimaterol drug residues, and achieve the effect of good immunogenicity
Pending Publication Date: 2020-05-15
XINXIANG UNIV
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- Abstract
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Problems solved by technology
[0003] The purpose of the present invention is to provide a kind of anti-cimaterol monoclonal antibody and application thereof, will solve the technical problem that the prior art cimaterol drug residue detects slower; question
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[0048] In order to make the technical means, innovative features, objectives and effects achieved by the present invention easy to understand, the present invention will be further explained below.
[0049] The embodiments described herein are specific specific implementations of the present invention, which are used to illustrate the concept of the present invention. They are all explanatory and exemplary, and should not be construed as limiting the embodiments of the present invention and the scope of the present invention. In addition to the implementation exceptions described here, those skilled in the art can also adopt other obvious technical solutions based on the content disclosed in the claims and specification of this application. These technical solutions include adopting any obvious changes to the embodiments described herein. Technical solutions for replacement and modification.
[0050] 1. Research background,
[0051] Preparation and identification of cimaterol artifi...
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Abstract
The invention provides a cimaterol-resistant monoclonal antibody and an application thereof, provides a method for preparing the cimaterol-resistant monoclonal antibody, and further provides a specific application of the cimaterol-resistant monoclonal antibody, wherein the method includes two contents of preparing a cimaterol antigen working solution and preparing a cimaterol monoclonal antibody,and the application includes preparation of an ELISA reagent kit or a colloidal gold chromatography test strip. The prepared complete antigen has good immunogenicity, an efficient stable monoclonal antibody is prepared, a standard solution after detection series dilution is obtained, and high-degree specificity of a monoclonal antibody and high sensibility of a method are organically combined, quick detection of the antigen is achieved, the cimaterol-resistant monoclonal antibody is suitable for detection of massive samples in a short time, and a base is established for detection of cimaterolremained in samples.
Description
Technical field [0001] The invention belongs to the field of biotechnology, in particular to an anti-cimaterol monoclonal antibody and its application. Background technique [0002] Cimaterol is a small molecule hapten (molecular weight of 219.28Da), which must be coupled with a carrier protein to become a complete antigen in order to stimulate the body to produce an immune response and induce the body to produce antibodies. Commonly used as carrier proteins for synthetic artificial antigens are: bovine serum albumin (BSA), ovalbumin (OVA), keyhole limpet hemocyanin (KLH), human serum albumin (HSA), synthetic polylysine Acid (PLL), etc., there are many commonly used coupling methods at present, and the choice of coupling method is affected by many aspects, such as the active group of the hapten, the binding site of the antigen and the antibody, and the cost of synthesis. Summary of the invention [0003] The purpose of the present invention is to provide an anti-cimaterol monoclo...
Claims
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IPC IPC(8): C07K16/44C07K14/77G01N33/577C12N15/06G01N33/535
CPCC07K16/44C07K14/77G01N33/577C12N15/02G01N33/535C07K2317/33
Inventor 王选年司艳芳岳锋郭东光齐永华潘鹏涛
Owner XINXIANG UNIV