Enzymolysis and extraction method of momordica charantia seed protein

Abstract

The invention relates to an enzymolysis and extraction method of a momordica charantia seed protein. The enzymolysis and extraction method comprises the following steps of (1) firstly drying momordicacharantia seeds, crushing the dried momordica charantia seeds which are not shelled into fine powder, and screening the fine powder for standby application; (2) weighing the momordica charantia seedfine powder, adding the weighed momordica charantia seed fine powder into water, performing ultrasonic treatment at room temperature, and performing heat preservation in water bath; (3) performing stirring while adding, and adjusting pH value through dilute hydrochloric acid; (4) adding pectinase and cellulase in material liquid, performing slow and uniform stirring, performing placing in the water bath, and performing standing; (5) performing stirring while adding, and adjusting the pH; (6) adding lipase, performing slow and uniform stirring, and performing standing in the water bath; (7) performing stirring while adding, adjusting the pH value of a solution to be 9.5, and performing standing; (8) centrifuging mixed liquor, taking supernatant, and performing filtration; (9) performing stirring while adding, adjusting the pH value of filtrate with the hydrochloric acid solution, and performing standing; (10) centrifuging the mixed liquor, discarding supernatant, and taking precipitate;(11) putting the precipitate on a semipermeable membrane, and washing away salt and impurities; and (12) freeze-drying the protein after impurity removal so as to obtain total protein of momordica charantia seeds.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural active substances, and in particular relates to an enzymatic extraction method of bitter gourd seed protein. Background technique [0002] Bitter melon (Momordica charantia L.) is an annual climbing herb of Cucurbitaceae Momordica genus. As a medicinal and edible plant, bitter melon has high nutritional value and therapeutic effect. In recent years, the planting area of ​​bitter gourd has gradually increased, and it has become the main vegetable variety and characteristic industry of "South Vegetable Transport North". Bitter melon seed protein is a natural polymer compound, rich in ribosome inactivating protein, charantin and other active ingredients, with health functions such as lowering blood sugar, anti-cancer, anti-virus, and enhancing immunity. Bitter melon seeds are a by-product of intensive processing of bitter melon, and are a high-quality vegetable protein resource with gr...

AI Technical Summary

Problems solved by technology

In recent years, there have been relatively few studies on bitter melon seed protein in my country, mainly focusing on the separation and purification of protein molecules and biological activities, and there are few reports on the extraction process of bitter melon seed protein.

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