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Compound microbial agent, and preparation method and application thereof to ginger

A technology of composite bacterial agents and microbial strains, applied in the field of microorganisms, to meet nutritional needs, promote growth, and increase dry and wet weights

Inactive Publication Date: 2020-05-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial compound bacterial agent of the present invention can effectively improve the soil structure, thereby solving the growth problem of ginger

Method used

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  • Compound microbial agent, and preparation method and application thereof to ginger
  • Compound microbial agent, and preparation method and application thereof to ginger
  • Compound microbial agent, and preparation method and application thereof to ginger

Examples

Experimental program
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preparation example Construction

[0055] In another specific embodiment of the present invention, the preparation method of the microbial composite inoculum comprises: mixing the Bacillus licheniformis LCDD6, Bacillus thuringiensis TA-201, Bacillus thuringiensis TA-202 and Bacillus mycoides TA-203 Mix in proportion.

[0056] In yet another specific embodiment of the present invention, the microbial composite inoculant can be used to promote crop growth. Described crop is specifically ginger, and concrete growth-promoting effect is shown as following any one or more:

[0057] 1) Enhance the root vitality of ginger;

[0058] 2) Promote the growth of ginger ground diameter;

[0059] 3) Increase the dry and wet weight of ginger stems and leaves;

[0060] 4) Increase the biomass of ginger.

[0061] In yet another specific embodiment of the present invention, a microbial composite preparation is provided, the active ingredient of the microbial composite preparation is a culture obtained by culturing the microbia...

Embodiment 1

[0082] Example 1: Strain identification

[0083] The characteristics of the colonies and cells of Bacillus licheniformis LCDD6, Bacillus thuringiensis TA-201, Bacillus thuringiensis TA-202 and Bacillus mycoides TA-203 in the microbial compound inoculum of the present invention are as follows: figure 1 Its physiological and biochemical indicators are shown in Table 1 below.

[0084] Table 1

[0085]

[0086] (“+” means positive, “-” means negative. For type strains, please refer to the Manual for the Identification of Common Bacterial Systems, Dongxiuzhu, etc.)

[0087] At the same time, by 16S rDNA identification, it was determined that the above strains were Bacillus licheniformis, Bacillus thuringiensis, Bacillus thuringiensis and Bacillus mycosis respectively.

Embodiment 2

[0088] Example 2: Growth-promoting test of compound biological inoculum ginger

[0089] Preparation of compound microbial inoculants: Four microbial strains of Bacillus licheniformis LCDD6, Bacillus thuringiensis TA-201, Bacillus thuringiensis TA-202 and Bacillus mycoides TA-203 stored on the slant were transferred to the liquid culture medium containing LB. In the test tube of the base, placed in a constant temperature shaker for 12 hours, temperature: 37°C, rotation speed: 180rpm; the activated bacterial solution was inserted into the LB liquid medium according to 1% inoculum, and placed in the shaker for 24 hours. Temperature: 37°C, rotation speed: 180 rpm, 4 kinds of microbial cells were cultured respectively to the late logarithmic growth phase. Bacillus licheniformis LCDD6, Bacillus thuringiensis TA-201, Bacillus thuringiensis TA-202 and Bacillus mycoides TA-203 (each bacterial solution OD 600 About 1.6), the four kinds of bacteria liquid are mixed according to the volu...

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Abstract

The invention provides a compound microbial agent, and a preparation method and an application thereof to ginger, and belongs to the technical field of microbes. The compound microbial agent at leastcomprises Bacillus lincheniformis LCDD6, Bacillus thuringiensis TA-201, Bacillus thuringiensis TA-202, and Bacillus mycoides TA-203. The compound microbial agent of the invention can provide nutritionfor the growth of ginger. The compound microbial agent of the invention is used to treat ginger, the root activity of ginger can be enhanced, the growth of a ground diameter of ginger can be promoted, and the dry and wet weight of ginger stems and leaves can be increased, so that the plant biomass is improved, the biological yield and quality of ginger are improved, and thereby the compound microbial agent has good practical application values.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial compound inoculant, a preparation method thereof, and an application on ginger. Background technique [0002] The information disclosed in this Background of the Invention is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Ginger, known as the "ancestor of dishes", is a commonly used seasoning plant in our daily life. It has high nutritional value. The spicy ingredient - zingerone, is an aromatic volatile oils. Moreover, the content of nutrients such as carotene and vitamin C contained in ginger is also extremely rich. Ginger also has certain medicinal value and is a common ingredient in Chinese medicine in my country. It has t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G22/25A01C1/00C12R1/10C12R1/01C12R1/07
CPCA01C1/00A01G22/25C12N1/20C12N1/205C12R2001/07C12R2001/075C12R2001/10
Inventor 丁延芹汪城墙杜秉海邴辉王文团姚良同刘凯
Owner SHANDONG AGRICULTURAL UNIVERSITY
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