Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glycyl proline dipeptidylaminopeptidase determining reagent kit

A kit and bisglycin peptide technology are applied in the direction of determination/inspection of microorganisms, measuring devices, instruments, etc., which can solve the problems of low measurement accuracy, unstable reagents, poor anti-interference ability, etc., and achieve low preparation cost, Enhanced stability and accuracy, the effect of improving accuracy

Active Publication Date: 2020-05-19
浙江夸克生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a glycylproline dipeptide aminopeptidase assay kit in order to overcome the defects of unstable reagents, low measurement accuracy and poor anti-interference ability in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glycyl proline dipeptidylaminopeptidase determining reagent kit
  • Glycyl proline dipeptidylaminopeptidase determining reagent kit
  • Glycyl proline dipeptidylaminopeptidase determining reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1 A kind of glycylproline dipeptide aminopeptidase assay kit

[0077]

Embodiment 2

[0078] Embodiment 2 A kind of glycylproline dipeptide aminopeptidase assay kit

[0079]

Embodiment 3

[0080] Embodiment 3 A kind of glycylproline dipeptide aminopeptidase assay kit

[0081]

[0082]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a glycyl proline dipeptidylaminopeptidase determining reagent kit, and relates to the technical field of biologic detection. The glycyl proline dipeptidylaminopeptidase determining reagent kit comprises a reagent R1 and a reagent R2 which are mutually independent, wherein the reagent R1 comprises the following ingredients by corresponding content of 5-10g / L trihydroxymethylaminomethane, 5-10g / L glycylglycine and 0.1-0.5g / L sodium azide, and the reagent R2 comprises the following ingredients by corresponding content of 10-20g / L glycyl proline para-nitroaniline tosylate,20-80ml / L ethanediol, 2-8g / L bovine serum albumin and 0.1-0.5g / L sodium azide. In an implementation process, the addition proportion of mannitol to mannan to glucosamine in the reagent R2 is (1-5) to(1-2) to 1, besides, the volume ratio of the reagent R1 to the reagent R2 is controlled to be (5-10) to 1, stability and accuracy of the reagents are reinforced, the capacity of resisting disturbanceof the reagents is increased, and further promotion and use of the reagents in the market can be facilitated.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a glycylproline dipeptide aminopeptidase assay kit. Background technique [0002] Glycylproline dipeptide aminopeptidase (GPDA) is distributed in body fluids such as liver, kidney, connective tissue, salivary glands and serum, and can specifically hydrolyze the peptide bond formed by proline at the second end of the peptide chain, The specific activity of the microsomes and soluble parts in the cells is significantly increased, and its physiological significance is mainly to hydrolyze the peptides from collagen in the blood. Therefore, the physiological effect of glycylproline dipeptide aminopeptidase may be related to the degradation of collagen peptides . [0003] As early as 1966, Hopsu-Havu and Glenner discovered a new dipeptide aminopeptidase in rat liver and kidney tissues. This enzyme hydrolyzes glycyl-prolyl-B-naphthylamine, releasing the dipeptide glycylpr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/37
CPCC12Q1/37G01N2333/948C12Q2337/12Y02P20/55
Inventor 商忆文章晓庆
Owner 浙江夸克生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com