Glycyl proline dipeptidylaminopeptidase determining reagent kit

Abstract

The invention discloses a glycyl proline dipeptidylaminopeptidase determining reagent kit, and relates to the technical field of biologic detection. The glycyl proline dipeptidylaminopeptidase determining reagent kit comprises a reagent R1 and a reagent R2 which are mutually independent, wherein the reagent R1 comprises the following ingredients by corresponding content of 5-10g / L trihydroxymethylaminomethane, 5-10g / L glycylglycine and 0.1-0.5g / L sodium azide, and the reagent R2 comprises the following ingredients by corresponding content of 10-20g / L glycyl proline para-nitroaniline tosylate,20-80ml / L ethanediol, 2-8g / L bovine serum albumin and 0.1-0.5g / L sodium azide. In an implementation process, the addition proportion of mannitol to mannan to glucosamine in the reagent R2 is (1-5) to(1-2) to 1, besides, the volume ratio of the reagent R1 to the reagent R2 is controlled to be (5-10) to 1, stability and accuracy of the reagents are reinforced, the capacity of resisting disturbanceof the reagents is increased, and further promotion and use of the reagents in the market can be facilitated.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a glycylproline dipeptide aminopeptidase assay kit. Background technique [0002] Glycylproline dipeptide aminopeptidase (GPDA) is distributed in body fluids such as liver, kidney, connective tissue, salivary glands and serum, and can specifically hydrolyze the peptide bond formed by proline at the second end of the peptide chain, The specific activity of the microsomes and soluble parts in the cells is significantly increased, and its physiological significance is mainly to hydrolyze the peptides from collagen in the blood. Therefore, the physiological effect of glycylproline dipeptide aminopeptidase may be related to the degradation of collagen peptides . [0003] As early as 1966, Hopsu-Havu and Glenner discovered a new dipeptide aminopeptidase in rat liver and kidney tissues. This enzyme hydrolyzes glycyl-prolyl-B-naphthylamine, releasing the dipeptide glycylpr...

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a glycylproline dipeptide aminopeptidase assay kit in order to overcome the defects of unstable reagents, low measurement accuracy and poor anti-interference ability in the prior art

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