Cytochrome detection method based on surface enhanced Raman

A surface-enhanced Raman and cytochrome technology, which is applied in the field of measuring the secondary structure of cytochrome, can solve the problems of complex operation, low sensitivity, large sample consumption, etc., and achieve the effect of simple operation method, simple operation process and simple operation

Pending Publication Date: 2020-05-19
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

Using these techniques, information on certain molecular levels of proteins can be measured, but these techniques have certain limitations
For example, X-ray crystallography depends on the crystallization state of proteins, and the crystallization process is likely to lead to denaturation of proteins; although nuclear magnetic resonance is widely used in the study of proteins, its sensitivity is relatively low, and the amount of samples used in experiments is large
In addition, the above method is complicated to operate, has high requirements on the quality of the personnel operating the instrument and the performance of the instrument itself, and the test cost is relatively high.

Method used

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  • Cytochrome detection method based on surface enhanced Raman
  • Cytochrome detection method based on surface enhanced Raman
  • Cytochrome detection method based on surface enhanced Raman

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Embodiment 1

[0022] Embodiment 1: This embodiment provides a method for detecting cytochromes based on surface-enhanced Raman, comprising the following steps:

[0023] Step 1, dissolving the cytochrome to be tested in water, preparing aqueous solutions with different concentration gradients and mixing them evenly;

[0024] Step 2, taking 2 μL of the solution obtained in step b and dripping it onto the silver nanorod array substrate to concentrate the solution to obtain a detection solution;

[0025] In step 3, the detection solution obtained in step 2 is subjected to SERS detection, the laser power is set to 30 mW, the integration time is 10 s, and the SERS spectrum of the detection solution is measured;

[0026] Step 4, according to the SERS spectrum of the detection solution obtained in Step 3, use Lorentz linear fitting to obtain structural information.

[0027] figure 1 The scanning electron microscope picture of the surface-enhanced Raman substrate used in Example 1 shows that the s...

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Abstract

The invention discloses a cytochrome detection and characterization method based on surface enhanced Raman spectroscopy (SERS). The method is used for directly collecting SERS signals of cytochrome and is simple to operate and label-free, and further analyzing to obtain information that the secondary structure of the cytochrome changes along with the concentration. A new method can be provided forreal-time monitoring of biological medicine and protein secondary structure changes.

Description

technical field [0001] The invention relates to a method for detecting cytochrome based on surface-enhanced Raman, in particular to a method for measuring the secondary structure of cytochrome. Background technique [0002] Cytochrome is a kind of electron transfer protein with iron porphyrin (or heme) as a prosthetic group, which is widely involved in redox reactions of animals, plants, yeast, aerobic bacteria, and anaerobic photosynthetic bacteria. Cytochrome is a good biological electron carrier, which passes through the reduced state of the iron atom in the heme prosthetic group (Fe 2+ ) and oxidation state (Fe 3+ ) The reversible change between realizes the transport of electrons. The porphyrin ring is connected to the iron atom with four valence bonds to form a four-ligand chelated complex, which is generally called heme. According to the different structures of heme prosthetic groups, cytochromes can be divided into A, B, C and D types. Taking the common cytochrom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 田康振聂新明田亚平
Owner XUZHOU NORMAL UNIVERSITY
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