Diatomite-cotton bollworm nucleopolyhedrosis virus composition and application thereof
A nuclear polyhedron, diatomite technology, applied in the field of agrochemical, can solve the problems of insecticidal instability, application limitation, mild pest damage, etc.
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[0044] A third aspect of the present invention provides a method for preparing the structured diatomaceous earth-cotton bollworm nuclear polyhedrosis virus wettable powder as described above, comprising the steps of:
[0045] Add the wetting agent and dispersant in the formula amount into the container, add - cotton bollworm nucleopolyhedrosis virus, stir and mix with it, then add the diatomaceous earth with specific structure, vibrate and mix.
[0046] Since the diatomite with a specific structure used in this application has a zigzag honeycomb structure, it is necessary to mix the additives uniformly before adding the diatomite with a specific structure. Mechanical pulverization cannot be used after adding the diatomite with a specific structure. The structure of the specific structure diatomite is prevented from being destroyed. When the auxiliary agent used is solid, weigh the appropriate auxiliary agent according to the designed formula, and carry out high-speed universal...
Embodiment 1
[0050] Example 1. Co-toxicity coefficient method (CTC) screening: combined toxicity determination of diatomaceous earth with specific structure and nuclear polyhedrosis virus of cotton bollworm to Spodoptera litura.
[0051] Referring to the method in the NY / T1154.14-2008 standard, the method of soaking leaf discs is adopted. Use a hole puncher with a diameter of 18mm to punch the cabbage leaves into leaf discs, immerse the leaf discs in the test drug solution for 10 seconds, and put them into a petri dish (90mm in diameter) with a moisture-retaining filter paper, and put 6 discs in each dish. After the leaf discs are dried naturally, select the 3rd instar larvae of Spodoptera litura that have been hungry for 4 hours and put them in the petri dish, with no less than 10 larvae in each dish, and repeat 4 times. After the treatment is completed, cover the dish lid and place it in the observation room to cultivate.
[0052] After 7 days, the death of the test insects was checked ...
Embodiment 2
[0060] Example 2. Co-toxicity coefficient (CTC) screening: joint toxicity determination of diatomaceous earth with specific structure and nuclear polyhedrosis virus of cotton bollworm to tea geometrids.
[0061] Referring to the method in the NY / T1154.14-2008 standard, the leaf soaking method is adopted. Select 3 buds of fresh tea trees, soak them in the test drug solution for 30 seconds, take them out to dry naturally, wrap the base of the buds with wet absorbent cotton to keep moisture, then seal the absorbent cotton balls with plastic wrap, put them in canned bottles, and The concentration was repeated 4 times. Then use a writing brush to insert lively and healthy 2nd instar nymphs of tea geometrids of the same size, 15 ± 2 heads per can, and seal them with white gauze. After processing, put it in the observation room.
[0062] After 7 days, the death of the test insects was checked and recorded. The standard for judging the death of the test insects was that the test ins...
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