Method for screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold

A technology of propionibacterium and screening methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effect of improving screening efficiency and accuracy

Pending Publication Date: 2020-05-22
EAST CHINA NORMAL UNIV +1
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  • Abstract
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  • Claims
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Problems solved by technology

At present, the research on antifungal active bacteria is mostly based on single strains, but there are few reports on the screening of combined strains with synergistic antifungal effects.

Method used

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  • Method for screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold

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Embodiment 1

[0056] Embodiment 1: have the screening of propionibacterium and lactic acid bacteria combined bacteria that synergistically inhibits Aspergillus flavus activity

[0057] (1) Cultivation of lactic acid bacteria seed liquid: pick out single colonies of 3 kinds of lactic acid bacteria (1#, 2#, 3#; respectively a strain of Lactobacillus rhamnosus, Lactobacillus plantarum and Lactobacillus paracasei), and insert In a 15mL screw-top test tube containing 15mL of MRS culture solution, culture at 30°C for 18 hours to obtain lactic acid bacteria seed solution.

[0058] (2) Cultivation of Propionibacterium seed solution: pick a single Propionibacterium colony, insert it into a 15mL screw-top test tube containing 15mL of SLB culture solution, and culture it at 30°C for 48 hours to obtain Propionibacterium seed solution .

[0059] (3) Preparation of Aspergillus flavus spore suspension: take the cultivated Aspergillus flavus slant, wash it with sterile water containing 0.01% Tween by volu...

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Abstract

The invention discloses a method for screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold. The method comprises the following steps:(1) preparing a lactic acid bacterium seed liquid and a propionibacterium seed liquid; (2) preparing a mold spore suspension; (3) adding the two bacterial liquids (a combined bacterium) of the step (1), single bacterial liquids (single bacteria) or a blank MRS (DeMan-Rogosa-Sharpe) medium into a porous plate; and (4) adding a solid medium into each pore, after curing, adding the mold spore suspension prepared in the step (2) into each pore, performing culture and observation, comparing mold growth situations of pores with the combined bacteria and pores of single bacteria, and screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold. The method is implemented on the porous plate, is capable of simultaneously screening multiple combined bacteria, is simple to operate, easy to implement and applicable to screening on combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold.

Description

technical field [0001] The invention belongs to the field of screening combination bacteria of propionibacterium and lactic acid bacteria, and relates to a screening method for combination bacteria of propionibacterium and lactic acid bacteria with synergistic antifungal activity. Background technique [0002] Mold plays an important role in food spoilage, with 5% to 10% of food production worldwide lost due to mold contamination. In addition, mycotoxins produced by some molds have toxicity such as carcinogenicity, mutagenesis, teratogenicity, neurotoxicity, nephrotoxicity, immunosuppression and estrogenic effects. [0003] As people pay more and more attention to food safety and health issues, efficient, healthy, safe and pollution-free natural microbial preservatives are gradually replacing traditional chemical preservatives, which is the development trend of the food industry. As the most representative probiotic species, lactic acid bacteria and propionibacterium have b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/18C12R1/25C12R1/225C12R1/67
CPCC12N1/20C12Q1/18
Inventor 贾彩凤冉琼鲍子旗刘玉婷董建华李超文潘建泽
Owner EAST CHINA NORMAL UNIV
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