Method for distinguishing individualized medication of losartan by mass spectrometry through detection products

Abstract

As different single nucleotide polymorphism (SNP) sites have extension primers with different molecular weights and multiple sites are detected by Mass Array matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), the invention can finally obtain a method for distinguishing the individualized medication of losartan by mass spectrometry through detection products. The method includes the steps of respectively designing multiple amplification primers and extension primers according to multiple to-be-detected target SNP sites; preparing a multiple amplification primer reaction system and an extension primer reaction system; simultaneously and respectively performing amplification and single base extension reaction on the multiple target SNP sites by usingmultiple sets of the primers in the reaction systems; and performing time-of-flight mass spectrometry on products after single base extension reaction, and identifying the genotypes of different drugmetabolism related SNP according to the products of the extension primers with different molecular weights represented by mass spectrum peaks so as to guide the medication of the antihypertensive druglosartan. The method can detect the SNP sites related to the metabolism of the antihypertensive drug losartan; and the method is low in cost, short in time consumption, simple and convenient in result analysis and extremely wide in application field and does not need to synthesize probes.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and relates to a method for detecting multiple PCR single-base extension products by using mass spectrometry characteristic peaks and its products. The method can simultaneously detect multiple PCR single-base extensions in two multiplex PCR reactions Amplified oligonucleotide products. More specifically, the method uses different time-of-flight mass spectrometry characteristic peaks generated by different target oligonucleotide fragments in the process of mass spectrometry typing, and simultaneously detects multiple target SNP sites to guide the antihypertensive drug Roxa Frank medication. Background technique [0002] Human genetic information is stored in the genome. In 2002, the Human Genome Project, an international cooperation project, was finally completed, drawing a fine map of the human genome structure and providing a reference sequence for related research. The human genome...

AI Technical Summary

Problems solved by technology

However, the method and kit provided by this invention patent can only detect polymorphic sites on the homocysteine ​​metabolism pathway, and cannot detect polymorphic sites not involved in homocysteine ​​metabolism , has limitations in clinical medication guidance

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