Application of Codonopsis pilosula oligosaccharide in preparation of medicine for preventing or treating gastrointestinal injury caused by anoxia

A technology of oligosaccharides and gastrointestinal tract, applied in the field of hypoxic drug screening, to relieve intestinal mucosal damage, enhance antioxidant levels, and improve inflammation

Active Publication Date: 2020-05-26
LANZHOU FOCI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the protection of Codonopsis oligosaccharides from hypoxia-i

Method used

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  • Application of Codonopsis pilosula oligosaccharide in preparation of medicine for preventing or treating gastrointestinal injury caused by anoxia
  • Application of Codonopsis pilosula oligosaccharide in preparation of medicine for preventing or treating gastrointestinal injury caused by anoxia
  • Application of Codonopsis pilosula oligosaccharide in preparation of medicine for preventing or treating gastrointestinal injury caused by anoxia

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Effect test

Embodiment 1

[0025] The preparation method of Codonopsis oligosaccharides is as follows: Codonopsis coarse powder and 95% ethanol are mixed, reflux extraction is carried out twice, each time for 1 hour, the filtrate and dregs are collected respectively, and the filtrate is concentrated under reduced pressure to obtain Codonopsis ethanol extract (CPEE) Add 10 times the weight of water to the collected dregs, decoct 3 times, 45 minutes each time, collect and combine the water extract, concentrate the water extract to 1 / 4 volume under reduced pressure, and obtain a concentrated solution; The solution is divided into two parts, one part of the concentrated solution is concentrated under reduced pressure again to obtain Codonopsis pilosula water extract (CPWE); the other part of the concentrated solution is added with ethanol to a final volume concentration of 80% for alcohol precipitation, and the precipitate is freeze-dried to obtain Codonopsis pilosula polysaccharide (CPPWE). ); the supernata...

Embodiment 2

[0027] Verification test:

[0028] In order to detect the protective effect of Codonopsis oligosaccharides on gastrointestinal injury induced by plateau hypoxia rats, the applicant of the present invention detected the intestinal protective effect of Codonopsis oligosaccharides on high altitude hypoxia rats. SD rats were randomly divided into 3 groups, 8 in each group, respectively blank control group (Control), high altitude hypoxia group (Model) and high altitude hypoxia + Codonopsis oligosaccharide group (CPO). The experiment was divided into two stages for 8 days: in the first 3 days, all rats in the group were placed in a normal environment; in the last 5 days, except for the blank group, the remaining rats were placed in an anoxic chamber at an altitude of 7000m for 5 days. During the experiment, the HCPO group was given Codonopsis oligosaccharides (100 mg / kg.bw) by intragastric administration every day, and the Control and HH groups were intragastrically administered eq...

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Abstract

The invention discloses application of Codonopsis pilosula oligosaccharide in preparation of a medicine for preventing or treating gastrointestinal injury caused by anoxia. The anoxia is plateau anoxia and/or tissue ischemic anoxia. The invention also provides a preparation method of the Codonopsis pilosula oligosaccharide. The Codonopsis pilosula oligosaccharide has the following advantages: themedicine prepared by adopting the application of the Codonopsis pilosula oligosaccharide in the preparation of the medicine for preventing or treating gastrointestinal injury caused by anoxia has a very good effect, is safe and effective, and has no toxic or side effect; experimental verification shows that the medicine containing the Codonopsis pilosula oligosaccharide can improve spleen atrophyand pulmonary emphysema caused by high-altitude anoxiaof rats, enhance the anti-oxidation level, improve inflammations, relieve intestinal mucosal damage and reduce EPO expression; and protein expression detection further shows that the Codonopsis pilosula oligosaccharide can reduce the expression of HIF-1 alpha in duodenum of a high-altitude hypoxic rat.

Description

technical field [0001] The invention relates to the technical field of screening drugs for hypoxia, in particular to the application of codonopsis oligosaccharides in the preparation of drugs for preventing or treating gastrointestinal damage caused by hypoxia. Background technique [0002] Long-term exposure to high-altitude hypoxic environments can have serious effects on various organs of the body. Hypoxia can lead to gastrointestinal inflammation, induce severe intestinal mucosal damage, promote the release of bacteria and endogenous toxins, and cause a series of inflammations. Codonopsis pilosula is a traditional Chinese medicine that nourishes qi and blood, and oligosaccharides of Codonopsis pilosula are its main active components, which have good immunoregulatory activity. However, the protection of oligosaccharides from Codonopsis pilosula against hypoxia-induced gastrointestinal damage has not been recorded in the prior art. Contents of the invention [0003] Th...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61K31/715A61P1/00A61K36/344
CPCA61K31/702A61K31/715A61K36/344A61P1/00
Inventor 胡芳弟白瑞斌樊精敏闫巧张菊李雪王燕萍
Owner LANZHOU FOCI PHARM CO LTD
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