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Polypeptide ligand Cy5-H8 for modifying quantum dots

A peptide ligand and quantum dot technology, applied in the field of peptide ligand Cy5-H8, can solve the problems of poor stability and slow binding speed, and achieve the effect of high stability, fast binding speed and good binding force

Active Publication Date: 2020-05-26
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of slow binding speed and poor stability between existing polypeptide ligands and QDs, provide a fast binding speed with QDs, and a new type of polypeptide ligand with good stability, and expand the popularity of QDs in the field of biomarkers with use

Method used

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  • Polypeptide ligand Cy5-H8 for modifying quantum dots
  • Polypeptide ligand Cy5-H8 for modifying quantum dots
  • Polypeptide ligand Cy5-H8 for modifying quantum dots

Examples

Experimental program
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Embodiment 1

[0039] The method of the present invention adopts the conventional solid-phase Fmoc method, that is, the monomer amino acid protected by Fmoc on the solid-phase resin is deprotected to expose the amino group, and forms a peptide bond with the carboxyl group of the amino acid in the solution through a condensation reaction, thereby linking the amino acid to the On the resin, the peptide chain is extended from the C-terminus to the N-terminus.

[0040] 1. Basic materials:

[0041] (1) Resin and linking molecule: The resin selected by the solid-phase Fmoc method is Rink Amide- resin. This kind of resin has very good swelling property, which can make the condensation reaction between the peptide chains better, and there is enough network space to meet the growing peptide chains. HBTU and HOBt are used as linking molecules to immobilize the polypeptide molecules on the resin.

[0042] (2) Monomer: The monomer used in the synthesis is a chemically modified α-amino acid.

[0043...

Embodiment 2

[0065] Refer to Example 1 for the steps of polypeptide synthesis. The sequence of the synthetic novel peptide ligand is as follows: Cy5-DDLVPRGSGK(H4)VK(H4)DPLPK(H4)LK(H4)G

[0066] According to the method of Example 1, the polypeptide sequence obtained in Example 2 and QDs were analyzed by capillary electrophoresis, the detection conditions were the same as in Example 1, and the test results found that it could be stably combined with QDs (such as Figure 7 ).

[0067] Electrophoresis conditions:

[0068] 25mM boric acid buffer (pH 9.3), the voltage is 20kV (λex=420nm. Solid line, λ em =605nm; dotted line, λ em = 665nm).

[0069] In the control experiment, the straight-chain linear ligand Cy5-DDGPLGVRGHHHHHH was combined with QDs, and the detection wavelength was 605 nm for the detection of QDs, and the dashed line was 665 nm for the detection of Cy5.

[0070] It can be seen from the above examples that the novel polypeptide ligand of the present invention can realize th...

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Abstract

The invention belongs to the technical field of nano-biology, and particularly discloses a polypeptide ligand (Cy5-H8) for modifying quantum dots. The polypeptide ligand comprises a dye Cy5 (1) used for generating FRET with quantum dots, a functional sequence (2), a histidine sequence (3) used for combining the quantum dots and a polypeptide folding sequence (4), and all the sequences are combinedthrough peptide bonds. The ligand is simple in synthesis method, is combined with the quantum dots through the histidine spacer sequence to greatly improve the combination rate and stability of the ligand and the quantum dots, and can be widely applied to biomarkers as a nano-fluorescent probe.

Description

technical field [0001] The invention belongs to the field of nano-biotechnology, and in particular relates to a polypeptide ligand Cy5-H8 for modifying quantum dots. Background technique [0002] Quantum dots (QDs), as one of the most popular and promising nanomaterials, possess many desirable properties, including their excellent size and / or material dependence; broad and continuous excitation spectra, narrow and symmetric emission spectra; high light and chemical stability. These unique optical properties enable QDs to be widely used in molecular detection, cell labeling, and in vivo imaging. [0003] In the application of biosensing and related targeted imaging, QDs need to be coupled with biomolecules (such as peptides, proteins, nucleic acids, etc.) to form various desired bioluminescent probes. In the method of QDs linking protein or polypeptide molecules, the metal affinity coordination between the Zn atoms on the surface of QDs and polyhistidine residues is directl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00C07K1/04C07K1/06
CPCC07K14/001Y02P20/55
Inventor 王建浩郑荣会邱琳兰敏贾文静周舒文崔朋飞雷晓玲王佳炜王璇
Owner CHANGZHOU UNIV