Test card for detecting glutamic-pyruvic transaminase by photochemical method and preparation method of test card

Alanine aminotransferase and test card technology, applied in the field of detection, can solve the problems of inability to be widely used and uneven test results, and achieve the effects of good matching, simple use, and avoiding pyruvate interference

Pending Publication Date: 2020-05-26
杭州联晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, several companies have related products on the market. Since the interference of endogenous pyruvate in the sample cannot be removed, the test results are uneven, so the market cannot be widely used. The market needs a pr

Method used

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  • Test card for detecting glutamic-pyruvic transaminase by photochemical method and preparation method of test card
  • Test card for detecting glutamic-pyruvic transaminase by photochemical method and preparation method of test card
  • Test card for detecting glutamic-pyruvic transaminase by photochemical method and preparation method of test card

Examples

Experimental program
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Effect test

preparation example Construction

[0045] A kind of preparation method of the test card that photochemical method detects alanine aminotransferase, comprises the steps:

[0046] Step 1: Prepare the first reaction solution according to the following formula,

[0047] The formula includes: L-alanine 50-500mM, pyruvate oxidase 50-200KU / L, α-ketoglutarate 5-100mM, peroxidase 50-200KU / L, thiamine pyrophosphate 0.1-1mM , magnesium chloride 0.1-5g / L, 4-aminoantipyrine 1-50mM, chromogen 1-50mM, film-forming agent 0.1-10g / L, stabilizer 1-50g / L, 0.1-2M buffer; Stir for one hour and set aside;

[0048] Step 2: Prepare the second reaction solution according to the following formula,

[0049] The formula includes: pyruvate oxidase 50-200KU / L, peroxidase 50-200KU / L, thiamine pyrophosphate 0.1-1mM, magnesium chloride 0.1-5g / L, 4-aminoantipyrine 1-50mM, Color developer 1-50mM, film-forming agent 0.1-10g / L, stabilizer 1-50g / L, 0.1-2M buffer;

[0050] Step 3: preparing the first reaction membrane 301,

[0051] Soak the reac...

Embodiment 1

[0058] Step 1: Prepare the first reaction solution: L-alanine 50mM, pyruvate oxidase 100KU / L, α-ketoglutarate 50mM, peroxidase 100KU / L, thiamine pyrophosphate 0.2mM, magnesium chloride 0.2g / L, 4-aminoantipyrine 10mM, MAOS10mM, sodium alginate 0.1-10g / L, seaweed number 1-50g / L, 0.05M phosphate buffer solution with pH=6; stir for one hour and wait use;

[0059] Step 2: Production of the first reaction film 301: soak the reaction film in the first reaction solution for 5 minutes, take it out, dry it at 45 degrees for 20 minutes, and set it aside;

[0060] Step 3: Take a polycarbonate single-sided adhesive 200 with a thickness of 0.1mm, paste the first reaction membrane 301 on the installation hole of the single-sided adhesive 200, and after pressing tightly, attach the MF1 polypropylene filter membrane 501 to the second blood filter On the membrane 401, paste the hydrophilic polyester mesh 601 directly above the MF1 polypropylene filter membrane 501, then cut a single strip and ...

Embodiment 2

[0062] Step 1: Prepare the first reaction solution: L-alanine 50mM, pyruvate oxidase 100KU / L, α-ketoglutarate 50mM, peroxidase 100KU / L, thiamine pyrophosphate 0.2mM, magnesium chloride 0.2g / L, 4-aminoantipyrine 10mM, MAOS10mM, sodium alginate 0.1-10g / L, trehalose 1-50g / L, 0.05M phosphate buffer solution with pH=6; stir for one hour before use ;

[0063] Step 2: Production of the first reaction film 301: soak the reaction film in the first reaction solution for 5 minutes, take it out, dry it at 45 degrees for 20 minutes, and set it aside;

[0064] Step 3: Take 0.1mm thick polycarbonate single-sided adhesive 200, paste the first reaction membrane 301 on the installation hole of the single-sided adhesive 200, and then place the cut second blood filter membrane 401 (modified polysulfone membrane PES 0.65) on the top of the first reaction membrane 301, press the edge tightly, then stick the first blood filter membrane (MF1 polypropylene filter membrane) 501 on the second blood fil...

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Abstract

The invention discloses a test card for detecting glutamic-pyruvic transaminase by a photochemical method and a preparation method of the test card. The test card comprises a test card upper shell, atest card lower shell, a main test hole, a contrast test hole, a sample adding hole, a diffusion film, a first blood filtering film, two second blood filtering films, a first reaction film, a second reaction film and a single-sided adhesive tape, wherein the formula of a first reaction solution comprises L-alanine, pyruvate oxidase, alpha-ketoglutaric acid, peroxidase, thiamine pyrophosphate, magnesium chloride, 4-aminoantipyrine, a color developing agent, a film forming agent, a stabilizer and a buffer solution; the formula of a second reaction solution comprises pyruvate oxidase, peroxidase,thiamine pyrophosphate, magnesium chloride, 4-aminoantipyrine, a color developing agent, a film forming agent, a stabilizer and a buffer solution; by using the test card and the preparation method provided by the invention, endogenous pyruvic acid interference can be avoided, and the detection result is well matched with the hospital inspection result.

Description

technical field [0001] The invention relates to the detection field, in particular to a test card for detecting alanine aminotransferase by photochemical method and a preparation method thereof. Background technique [0002] Alanine aminotransferase (ALT) mainly exists in various cells, especially in liver cells. The content of transaminase in the whole liver is about 100 times that in blood. Normally, as long as a small amount is released into the blood, the enzyme activity in the serum can be significantly increased. In the acute stage of various viral hepatitis and drug poisoning liver cell necrosis, ALT is released into the blood in large quantities, so it is an important indicator for diagnosing viral hepatitis and toxic hepatitis. As long as 1% of liver cells are necrotic, the activity of enzymes in the blood can be doubled, so transaminases (especially ALT) are sensitive signs of acute liver cell damage. [0003] The detection of alanine aminotransferase is now main...

Claims

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Application Information

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IPC IPC(8): C12Q1/52
CPCC12Q1/52
Inventor 陈军刚
Owner 杭州联晟生物科技有限公司
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