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Bambusa multiplex transcription factor BmbZIP62 gene and application thereof

A transcription factor and gene technology, applied in the filial piety bamboo transcription factor BmbZIP62 gene and its application field, can solve problems such as new functions to be verified

Active Publication Date: 2020-05-29
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological functions of many bZIP transcription factors have been clarified, there are still some functional new genes to be developed, or new functions of known functional genes to be verified

Method used

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  • Bambusa multiplex transcription factor BmbZIP62 gene and application thereof
  • Bambusa multiplex transcription factor BmbZIP62 gene and application thereof
  • Bambusa multiplex transcription factor BmbZIP62 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Acquisition of gene encoding BmbZIP62 transcription factor in filial bamboo

[0026] 1. RNA extraction

[0027] The extraction material was bamboo shoots of filial piety bamboo. RNA was extracted using the Yuanping Hao RNAPure Ultra-Pure Rapid Extraction Kit, extracted according to the operating instructions, and stored at -80°C for later use.

[0028] 2. cDNA first-strand synthesis and reverse transcription PCR

[0029] The total RNA was reverse-transcribed into cDNA using the Whole Type Gold (Beijing) one-step cDNA synthesis kit, following the operating instructions. Reaction system and reaction conditions are: 2 μg prepared total RNA, Anchored Oligo(dT) 18 Primer 1μL, 10μL 2×TS Reaction Mix, 1μL TransScript RT / RI Enzyme Mix, 1μL gDNARemover, add RNase-free Water to make up to 20μL, mix gently and centrifuge. Reverse transcription at 42°C for 30 minutes in a PCR instrument, 5s at 85°C; cool on ice; store at -20°C after centrifugation.

[0030] 3. PCR am...

Embodiment 2

[0033] Example 2: Functional Analysis of BmbZIP62 Transcription Factor of Filial Bamboo

[0034] 1. Sequence comparison analysis

[0035] In order to analyze the genetic relationship between BmbZIP62 of filial bamboo and bZIP transcription factors in model plants Arabidopsis and rice, a phylogenetic tree of BmbZIP62 and bZIP transcription factors in other species was established using MEGA7. result( figure 2 ) indicated that BmbZIP62 had the closest relationship with rice OsbZIP62 and OsbZIP29 transcription factors.

[0036] The amino acid sequence homology of BmbZIP62 and its closest relatives OsbZIP62 and OsbZIP29 was analyzed by DNAMAN8. Analysis results such as image 3 As shown, the sequence similarity between BmbZIP62 and OsbZIP62 is 61.27%, and the sequence similarity between BmbZIP62 and OsbZIP29 is 47.97%.

[0037] 2. Arabidopsis transgene analysis

[0038] 2.1 Vector construction

[0039] The PCR product whose sequencing sequence was consistent with the transc...

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Abstract

The invention discloses a bambusa multiplex transcription factor BmbZIP62 gene and application thereof, and belong to the technical field of plant genetic engineering. The transcription factor BmbZIP62 provided by the bambusa multiplex transcription factor BmbZIP62 gene and application thereof belongs to the basic-leucine zipper transcription factor family, and a nucleotide sequence of the transcription factor BmbZIP62 is shown as in SEQ ID NO.1, an amino acid sequence of encoded protein is shown as in SEQ ID NO.2. The bambusa multilex transcription factor BmdZIP62 gene and application thereofreveal the systematic evolutionary status of the bambusa mutiplex transcription factor BmdZIP62 encoding gene by gene cloning expression and comparative analysis, overexpression in Colombian Arabidopsis thaliana plants can change leaf shapes and shorten petioles of rosette leaves of the plants; and overexpression in rice can dwarf plants. In addition, a method for applying the gene is further provided, and the gene plays an important role in studying the development mechanism of plant dwarfing, leaf shape changing and petiole shortening.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a transcription factor BmbZIP62 gene of filial bamboo (Bambusa multiplex) and an application thereof. Background technique [0002] The bZIP (basic leucine zipper motif) transcription factor is one of the most widely distributed and conserved transcription factors in eukaryotes. The A subfamily is mainly involved in the regulation and expression of ABA and environmental stress, the C subfamily is mainly involved in seed development and environmental stress, the D subfamily is involved in the regulation of antioxidant and pathogen defense, and the B, F, E, I and other subfamilies There are few reports (Jakoby et al., Trendsin Plant Science, 7(3), 106-111, 2002), members of this family participate in the regulation of various biological processes of plant growth and development. Although the biological functions of many bZIP transcription factors have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46A01H6/20
CPCC07K14/415C12N15/8261
Inventor 魏强郭琳丁雨龙
Owner NANJING FORESTRY UNIV
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