Tissue culture and rapid propagation method for xanthostemon chrysanthus

A golden apple, tissue culture technology, applied in the field of rapid propagation of golden apple, can solve the problems of long bud induction period, large amount of auxin and mitogen, and achieve the effect of reducing the amount of hormones and improving the effect of proliferation

Active Publication Date: 2020-06-02
钦州市林业科学研究所
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amount of auxin and mitogen added in the above method is relatively large and the bud induction period is long

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture and rapid propagation method for xanthostemon chrysanthus
  • Tissue culture and rapid propagation method for xanthostemon chrysanthus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Selection of explants: in early spring, cut the semi-lignified new shoots with latent buds of the current year from the blooming golden apple tree as explants; Dilute oxazolone EC by 2000 times and spray the whole plant once.

[0023] (2) Disinfection of explants: Cut off the leaves on the branches, rinse them with tap water for 10-15 minutes, and then wash them with a soft-bristled toothbrush and an appropriate amount of detergent. When washing, use a soft-bristled toothbrush to scrub the branches 2-3 times; Rinse with sterilized water 4 to 5 times, then cut the branches into stems with 1 to 2 axillary buds, transfer them to the ultra-clean workbench and put them in a sterilized container, and wash them with 0.1% HgCl 2 Soak for 8-12 minutes, and finally rinse 4 times with sterilized water for later use.

[0024] (3) Axillary bud induction: Use tweezers to transfer the above-mentioned sterilized stem segments to the inoculation dish, cut the morphological lower en...

Embodiment 2

[0040] The aseptic buds obtained by the method described in Example 1 are tested by multiplying, rooting, seedling hardening and transplanting in "Jinappa Tissue Culture Rapid Propagation Technology" (Chen Qiming, "Forestry Survey and Design" 2019 02 issue), details as follows:

[0041] (4) Proliferation culture: the aseptic buds were cut into small sections according to the internodes, inserted obliquely in the proliferation medium and cultivated for 25 days to obtain clustered buds. The formula of the proliferation medium is: MS+6-BA 0.6 mg / L+NAA 0.3 mg / L+IBA 0.1 mg / L+riboflavin 1 g / L+sugar 30 g / L+carrageenan 5.8 g / L. Proliferation culture conditions are: temperature 24°C, light 1000lx, light time 14h / d, humidity 70%.

[0042] (5) Rooting culture: a single plant of clustered shoots was cut and inoculated on WPM medium for 7 days, and then transferred to rooting medium for 10 days to obtain rooted shoots. The formula of the rooting medium is: MS+IBA0.6mg / L+ABT 0.2mg / L+white...

Embodiment 3

[0045] Adopt the aseptic bud that the method described in Example 1 obtains to continue to cultivate.

[0046] (4) Proliferation culture: the aseptic buds were cut into small sections according to the internodes, inserted obliquely in the proliferation medium and cultivated for 25 days to obtain clustered buds. The formula of the proliferation medium is: improved WPM, 0.03 mg / L of 6-BA, 0.01 mg / L of NAA, 1.0 mg / L of L-leucine, and 40 g / L of tomato juice. Proliferation culture conditions are: temperature 24°C, light 1000lx, light time 14h / d, humidity 70%.

[0047] (5) Rooting culture: a single plant of clustered shoots was cut and inoculated on WPM medium for 7 days, and then transferred to rooting medium for 10 days to obtain rooted shoots. The formula of the rooting medium is: 3 / 4 improved WPM, NAA 0.5-1.0 mg / L. The rooting culture condition is: temperature 23 ℃, light intensity 1000lx, light time 10h / d, humidity 70%.

[0048] (6) Seedling hardening and transplanting: the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture and rapid propagation method for xanthostemon chrysanthus. The tissue culture and rapid propagation method comprises the steps of explant disinfection, axillary bud induction, multiplication culture and rooting culture, wherein the step of axillary bud induction is as follows: inoculating a disinfected explant into an axillary bud induction culture medium,and carrying out culturing for 18-22 days so as to obtain sterile buds, wherein the axillary bud induction culture medium comprises the following components: improved WPM, 0.01 to 0.03 mg / L of 6-BA, 0.01 to 0.02 mg / L of NAA, 1.0 to 2.0 mg / L of L-leucine and 40 to 60 g / L of tomato juice. According to the invention, the improved WPM culture medium is combined with the leucine and the tomato juice, so the usage amount of hormone in an axillary bud induction period can be greatly reduced; meanwhile, the axillary bud induction period can be shortened; and the cost is reduced.

Description

technical field [0001] The invention belongs to a plant tissue culture propagation method, in particular to a tissue culture rapid propagation method of golden apple. Background technique [0002] Golden apple fruit (Xanthostemon chrysanthus F.Muell.ex Benth), is an evergreen small tree of the family Myrtaceae (Myrtaceae), native to Australia, and is one of the representative plants of the Australian tropical rainforest. ) city flower, introduced to Fujian, Guangdong and other places in recent years. Golden apple leaves are opposite, alternate or clustered, lanceolate, entire, leathery, smooth leaf surface, guava smell after rubbing, new leaves are red. The inflorescences are spherical, blooming terminally or in the axils of branches and leaves. The color is yellow-green when it first blooms, and turns yellow with time, and is golden yellow when it is about to wither. Although it is a flower, its petals have actually degenerated, with only obvious round sepals and stamens ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/005A01H4/001Y02A40/22
Inventor杨利平时群李芳菲杨琼陈丽文吴红英李清香劳赞新余小红谭冬晓韦冬玲
Owner钦州市林业科学研究所