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Silicon bead method nucleic acid extraction equipment and use method and application thereof

An extraction equipment and a technology of silicon bead method, which is applied in the field of nucleic acid detection, can solve problems such as the difficulty of implementation and the inability to achieve solid-liquid separation through magnetic attraction, and achieve the effect of improving extraction efficiency

Pending Publication Date: 2020-06-02
广州高盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In contrast, silicon bead particles are not magnetic, and solid-liquid separation cannot be achieved through magnetic attraction. Therefore, the automatic nucleic acid extraction process based on the silicon bead method is more difficult to implement, while the real automatic extraction of silicon beads is No report

Method used

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  • Silicon bead method nucleic acid extraction equipment and use method and application thereof
  • Silicon bead method nucleic acid extraction equipment and use method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] In this embodiment, the silicon bead nucleic acid extraction equipment is used to extract the nucleic acid in the test material.

[0083] (1) Add 10 μL of frozen venous blood sample diluted 1000 times onto the cotton swab head and put it into the lysis plate of the reaction module, transfer the lysate to the lysis plate using a pipette pump, and the test material The total volume of the lysate and the lysate is 400 μL, and the lysate is carried out at a shaking speed of 350 rpm, a heating temperature of 56°C, and a time of 30 min. After the end, centrifuge with a centrifugal module at a speed of 2000 g and a time of 5 min. Transfer to the binding plate of the reaction module;

[0084] (2) Use a pipette pump to put the adsorption liquid and silicon beads into the binding plate. The total volume of the adsorption liquid and silicon beads is 570 μL, and then transfer the binding plate to the heating and shaking module for adsorption. Shake at 1050rpm for 1min, stand still...

Embodiment 2

[0088] The difference from Example 1 is that the shaking speed is 800 rpm during cracking in step (1), and all the other steps and operation methods are the same as in Example 1.

Embodiment 3

[0090] The difference with Example 1 is that the adsorption time in step (2) is 30min, and all the other steps and operating methods are the same as in Example 1.

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Abstract

The invention provides silicon bead method nucleic acid extraction equipment and a use method and application thereof. The silicon bead method nucleic acid extraction equipment includes a reaction module, a heating shaking module, a centrifugal module and pipetting pump; the reaction module includes a lysis plate and a binding plate, the lysis plate is used to lyse a test material to be tested, and the binding plate is used to complete the binding and release of nucleic acid and silicon beads; the heating shaking module is used for heating shaking the binding plate; the centrifugal module is used for centrifugal precipitation; and the pipetting pump includes a pipetting arm and a suction head, and is used to move the lysis plate, the binding plate and a reagent. According to the silicon bead method nucleic acid extraction equipment, high-speed centrifugation can be realized, silica beads are effectively precipitated, solid-liquid separation is realized, residual liquid in an adsorptionstep and a cleaning step is effectively controlled, and the amount of the residual liquid is less than 5 [mu]L; meanwhile, automatic extraction of nucleic acids is realized, the amount of sample processing is large, the extraction time is short, nucleic acid extraction personnel, especially forensics, can be assisted with improving of efficiency, and the foundation is laid for subsequent DNA testing.

Description

technical field [0001] The present invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid extraction device and its use method and application, in particular to a silicon bead method nucleic acid extraction device and its use method and application. Background technique [0002] Nucleic acid extraction and detection technology is widely used in the field of forensic medicine. Forensic medical samples are lysed to release nucleic acid DNA, and then specifically bind to magnetic beads or silicon beads in a specific solution environment, and use alcohol-containing solution for rinsing and purification, and finally change the solution environment to make DNA from magnetic beads or silicon beads release, so as to achieve the purpose of extraction and purification. With the advancement of nucleic acid testing technology, DNA extraction has become a key link affecting the success of DNA testing. [0003] With the increasing demand for ...

Claims

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Application Information

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IPC IPC(8): C12M1/02C12M1/00C12Q1/6806
CPCC12Q1/6806C12Q2523/308C12Q2523/32
Inventor 罗深恒黄梓辉刘柏添
Owner 广州高盛生物科技有限公司
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