Method for detecting sulfonamide antibiotics in water

Abstract

The invention discloses a method for detecting sulfonamide antibiotics in water. The method comprises the following steps: water sample filtering: selecting a water sample, and enabling the water sample to pass through a 0.45 mu m nitrocellulose filter membrane to remove suspended particles in the water; chromatographic determination: the mass spectrometry conditions are as follows: the specification of a Symmetry C18 chromatographic column is 150mm*2.1 mm and 3.5 mu m, the mobile phase is 0.1% formic acid solution and methanol solution, the column temperature is 30 DEG C, the flow rate is 0.25 mL / min, and the sample injection amount is 10 mu L; determining and analyzing; formulating a standard intermediate solution; and carrying out calculating and analyzing: samples with various concentrations in the linear range are continuously injected for three times, the peak area of the sample solution is calculated according to the drawn chromatogram, the relative standard deviation of the peak area is calculated, the detection limit is calculated by adopting a three-time signal-to-noise ratio, and the lower limit of quantification is calculated by adopting a 10-time signal-to-noise ratio.The method has the advantages that the detection efficiency is high, eight sulfonamide antibiotics can be detected at a time, the selectivity is high, the application range is wide, and the operationmethod is simple, convenient and rapid.

Description

technical field [0001] The invention relates to the technical field of antibiotic analysis, in particular to a method for detecting sulfonamide antibiotics in water. Background technique [0002] Antibiotics (SAs) are one of the most widely used drugs at present, and have been detected in many environmental water bodies, such as waste water (sewage) water, surface water, ground water, and even drinking water. Antibiotics are one of the earliest and most widely used drugs, mainly used to prevent and treat bacterial and fungal infectious diseases. According to relevant reports on sulfa antibiotics in water from the United States, Canada, and some countries in the European Union, the concentration of sulfa antibiotics in water is usually maintained at a concentration level of micrograms per liter or nanograms per liter. At present, there are also reports about antibiotic residues in some surface waters in my country. Although the half-life of most antibiotics is not long, beca...

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Problems solved by technology

[0005] In order to solve the technical problems of low sensitivity and inability to realize rapid and accurate analysis of sulfa antibiotics in water in the prior art, the present invention provides a detection method for sulfa antibiotics in water to solve the above technical problems

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