Western China cattle genome selection method

A genome selection, Huaxi cattle technology, applied in the fields of genomics, biochemical equipment and methods, proteomics, etc., can solve problems such as insufficient seed production and supply capacity, and achieve the effect of saving breeding costs and improving selection efficiency.

Active Publication Date: 2020-06-05
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

my country's beef cattle industry currently lacks the ability to produce and supply seed

Method used

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  • Western China cattle genome selection method

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Experimental program
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Effect test

Embodiment 1

[0030] Huaxi cattle genome selection method, the specific steps are as follows:

[0031] Step 1: Construct a reference population and measure economic traits: A team has built a basic cow herd of Huaxi cattle with 2,320 heads in the Ulagai management area of ​​Xilin Gol League, Inner Mongolia since 2008. The number has exceeded 4000. The offspring of this basic cow group were used to construct the reference group of Huaxi cattle, and the measured phenotypic data included 87 important economic traits in 6 categories: growth and development, fattening, slaughter, carcass, meat quality, and reproduction;

[0032] Step 2: Collect blood from each cow in the reference group, extract DNA, use Illumina BovineHD (770k) high-density chip for genotyping, and use plink 1.0 software to process and quality control the data after typing;

[0033] Step 3: Using the sequencing data of 44 cows, use Beagle software to fill the reference group 770K chip data into the sequencing data (16165263SNP...

Embodiment 2

[0044] Step 1. Establishment of reference populations and determination of phenotypic traits: Huaxi cattle resource population established by a team. The team has built my country’s first Huaxi cattle resource population in the Ulagai management area of ​​Xilingol League, Inner Mongolia since 2008. Year by year Expand the herd. By 2017, the number of basic cows has exceeded 4,000. In July or August each year, measure the body size and body weight data of calves and basal cows in the Ulagai management area, and collect corresponding breeding record data. In order to facilitate the measurement and collection of data on growth and development traits, slaughter traits, carcass traits and meat quality traits of resource groups in the later stage, Huaxi cattle aged 5 to 9 months in the Uragai area are transported to a fattening and slaughtering integrated company in October every year. When cattle enter the field, they must measure the entry weight and body size data. During intensi...

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Abstract

The invention discloses a Western China cattle genome selection method, and aims to solve the problem of insufficient seed production and supply capacity of existing beef cattle breeding groups. The method comprises the following specific steps: 1, constructing a reference population and determining economic traits; 2, performing genotyping on the reference population, and performing processing and quality control on data; 3, performing genotype data filling to obtain sequencing data, and then performing QTL locus screening; 4, calculating effect values of all SNP markers of each character inthe reference population; and 5, obtaining a candidate population genome breeding value, and calculating the comprehensive selection index of individuals according to the genome estimated breeding value of each character. The invention aims to establish the efficient and high-quality breeding method for the Chinese western cattle based on the genome selection technology, comprehensively improve the beef cattle population selection efficiency in China, greatly save the breeding cost, provide a molecular breeding method for the breeding of the high-quality Chinese western cattle, and promote therapid development of the beef cattle breeding industry.

Description

technical field [0001] The invention relates to the field of beef cattle breeding, in particular to a genome selection method for Huaxi cattle. Background technique [0002] Breed and provenance are the primary factors limiting the development of my country's beef cattle industry. The existing beef cattle breeding group in my country's beef cattle industry is not capable of producing and supplying seeds, and the breeding bulls mainly rely on foreign imports. Among the overall stock of beef cattle, there are 27.88 million Huaxi crossbred cattle, accounting for 65% of the stock of beef cattle. There are currently 38 breeding bull stations in China that involve the breeding of meat bulls, with 1,900 semen-collecting bulls in stock, including 1,100 Huaxi cattle. The breeding group can only provide about 100 qualified bulls every year, and about 250 new bulls need to be imported every year. , the import rate is as high as 70%. Therefore, people are also doing related research....

Claims

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Application Information

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IPC IPC(8): G16B20/30G16B20/20G16B35/20
CPCG16B20/30G16B20/20G16B35/20C12Q1/6827G16B20/40C12Q2537/165
Inventor 李俊雅朱波高会江高雪张路培徐凌洋陈燕蔡文涛
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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